Recipe for standard BG-11 media v1

Anna Behle, Alice Pawlowski


Stanier RY, Deruelles J, Rippka R, Herdman M, Waterbury JB: Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria. Microbiology 1979, 111:1–61. Recipes for standard and alternative BG11 for culturing freshwater cyanobacteria, such as Synechocystis sp. PCC 6803, as described. Media is usually not suitable for marine cyanobacteria. Final Concentration of Medium. CaCl2*2 H2O 0.036 g/L Citric acid 0.006 g/L NaNO3 1.4958 g/L MgSO4* 7 H2O 0.0749 g/L 0.25M Na2EDTA (pH 8) 0.0056 mL/L Na2CO3 20 µg/ml Fe(III) Ammonium citrate 6 µg/ml K2HPO4 * 3H2O 30 µg/ml TES Buffer (pH 8) 10 mM H3BO3 2.86 mg/L MnCl2 * 4 H2O 1.81 mg/L ZnSO4 * 7 H2O 0.222 mg/L Na2MoO4 * 2 H2O 0.390 mg/L Co(NO3)2 *6 H2O 0.049 mg/L (CuSO4 * 5 H2O 0.079 mg/L if required)

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