Reciprocal interactions between cancer and Schwann cells contribute to oral cancer progression and pain

Heliyon
Elizabeth SalvoYi Ye

Abstract

Pain associated with oral squamous cell carcinoma (oral SCC) decreases quality of life and survival. The interaction between cancer and the peripheral nerves is known to initiate and amplify pain and contribute to carcinogenesis. Schwann cells envelop peripheral nerves and are activated in response to neuronal damage. The contributions of Schwann cells to oral SCC progression and pain are unknown. Using a non-contact co-culture model, we demonstrate that Schwann cells (RSC-96) and oral SCC cells (HSC-3) reciprocally interact to promote proliferation, migration, and invasion. Schwann cell-oral SCC interaction leads to increased production of adenosine, which stimulates cell proliferation and migration of both cell types. The adenosine receptor A2B (ADORA2B) is expressed on RSC-96 cells. We show that supernatant from the RSC-96 cells co-cultured with HSC-3 cells induces increased mechanical hypersensitivity in mice compared to supernatant from control RSC-96 cells. Treatment with the ADORA2B antagonist PSB603 significantly inhibits co-culture interactions - proliferation and migration, and co-culture supernatant induced mechanical hypersensitivity. RSC-96 cells co-cultured with HSC-3 cells secrete increased amounts of the pronoci...Continue Reading

Citations

Sep 29, 2020·Frontiers in Oncology·Aysha FerdoushiHubert Hondermarck
Jan 8, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael R ShurinYuri L Bunimovich
Jan 21, 2021·Scientific Reports·Elizabeth SalvoYi Ye
Feb 6, 2020·Current Opinion in Otolaryngology & Head and Neck Surgery·Zuzana SaidakAntoine Galmiche
Jun 3, 2021·International Journal of Molecular Sciences·Yi YeJennifer Gibbs

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Methods Mentioned

BETA
PCR
Assay
protein assay

Software Mentioned

Nikon Element
Image Lab
RTCA
GraphPad Prism
Nikon TI Element

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