Abstract
Butyrate, a product of colonic bacterial flora, functions as an antiproliferative agent and induces cell differentiation in a variety of cell types. In the present study, the effects of butyrate on cell growth and expression of alpha-fetoprotein (AFP) and albumin genes in HuH-7 human hepatoma cells were investigated. The HuH-7 cells were treated with sodium butyrate (0-1 mmol/L), and numbers of viable cells were counted at 24, 48, and 72 hours after treatment. To elucidate the effects of sodium butyrate on AFP and albumin gene expression, Northern blotting and transient chloramphenicol acetyltransferase plasmid transfection experiments were performed. Cell growth was dose dependently inhibited by sodium butyrate. By Northern blot analysis, the level of AFP messenger RNA was reduced by treatment with sodium butyrate, whereas the level of albumin messenger RNA was elevated by this treatment. In transient chloramphenicol acetyltransferase plasmid transfection experiments, sodium butyrate repressed the AFP promoter activity but did not change the AFP enhancer or silencer activities. In contrast, the albumin promoter activity was stimulated by sodium butyrate. These results suggest that butyrate leads to the reciprocal differentiati...Continue Reading
Citations
Mar 3, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T NikiA Geerts
Feb 24, 2001·Journal of Dental Research·T Kurita-OchiaiK Fukushima
Jun 12, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·D SunG Y Minuk
May 15, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H SaitoH Ishii
May 31, 2001·Journal of Cellular Physiology·H EbinumaH Ishii
Feb 27, 2013·International Journal of Molecular Medicine·Hong-Gang WangGuo-Zhong Ji
Jan 26, 2013·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Kazuhiko Nakao, Tatsuki Ichikawa
Apr 9, 2015·The Journal of Biological Chemistry·Huan HeZhihua Liu
Aug 21, 2020·The Cerebellum·S WoelkeS Zielen
Feb 24, 1999·Journal of Gastroenterology and Hepatology·K N KhanY Kato
Sep 7, 1999·Gene Therapy·H IshikawaK Eguchi
Sep 25, 1999·The Journal of Biological Chemistry·K NakaoN Ishii
Aug 16, 2001·Neurosurgery·N ItoI Saito
May 1, 1997·The Journal of Nutrition·G A WeaverM J Wolin
Apr 16, 2002·Infection and Immunity·Tomoko Kurita-OchiaiKazuo Fukushima
Jan 22, 2005·Cellular Microbiology·Jennifer ByersDaniel Eichinger
Jul 21, 2017·Cell Death & Disease·Libin WeiLin Yang
Apr 15, 1999·Seminars in Cancer Biology·G I Abelev, T L Eraiser
Mar 6, 1999·Experimental Cell Research·F LallemandJ Mester
Jun 12, 1999·Journal of Hepatology·H MazumeK Eguchi
May 4, 2001·Biochimica Et Biophysica Acta·K TanakaM Yamamoto
Feb 15, 2000·Journal of Hepatology·M ZhangG Y Minuk
Mar 7, 2001·Gastroenterology·R A Bras-GonçalvesM F Poupon