PMID: 8962097Dec 10, 1996Paper

Reciprocal regulation of Gs alpha by palmitate and the beta gamma subunit

Proceedings of the National Academy of Sciences of the United States of America
T IiriH R Bourne

Abstract

Hormonal activation of Gs, the stimulatory regulator of adenylyl cyclase, promotes dissociation of alpha s from G beta gamma, accelerates removal of covalently attached palmitate from the G alpha subunit, and triggers release of a fraction of alpha s from the plasma membrane into the cytosol. To elucidate relations among these three events, we assessed biochemical effects in vitro of attached palmitate on recombinant alpha s prepared from Sf9 cells. In comparison to the unpalmitoylated protein (obtained from cytosol of Sf9 cells, treated with a palmitoyl esterase, or expressed as a mutant protein lacking the site for palmitoylation), palmitoylated alpha s (from Sf9 membranes, 50% palmitoylated) was more hydrophobic, as indicated by partitioning into TX-114, and bound beta gamma with 5-fold higher affinity. beta gamma protected GDP-bound alpha s, but not alpha s-GTP[gamma S], from depalmitoylation by a recombinant esterase. We conclude that beta gamma binding and palmitoylation reciprocally potentiate each other in promoting membrane attachment of alpha s and that dissociation of alpha s.GTP from beta gamma is likely to mediate receptor-induced alpha s depalmitoylation and translocation of the protein to cytosol in intact cells.

References

Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T L JonesA M Spiegel
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·L A RansnäsP A Insel
Apr 21, 1995·The Journal of Biological Chemistry·P T Wilson, H R Bourne
Jan 13, 1995·The Journal of Biological Chemistry·P B WedegaertnerH R Bourne
Jul 1, 1994·Cell·P B Wedegaertner, H R Bourne
Apr 1, 1994·Current Opinion in Cell Biology·P J Casey
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·S M MumbyA G Gilman
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M E LinderS M Mumby
Jan 5, 1996·The Journal of Biological Chemistry·J R HeplerA G Gilman
Jan 25, 1996·Nature·D G LambrightP B Sigler
Mar 22, 1996·The Journal of Biological Chemistry·J T DunphyM E Linder
Sep 20, 1996·The Journal of Biological Chemistry·J A Duncan, A G Gilman
Aug 1, 1996·Molecular Biology of the Cell·P B WedegaertnerM von Zastrow

❮ Previous
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Citations

Apr 11, 2006·Virus Genes·Tetsuo KoshizukaYukihiro Nishiyama
Dec 5, 1998·Biochimica Et Biophysica Acta·J T Dunphy, M E Linder
Dec 21, 2002·Pharmacology & Therapeutics·Riad Qanbar, Michel Bouvier
Mar 24, 2000·Progress in Lipid Research·G A Thompson, H Okuyama
Dec 24, 1998·Molecular and Cellular Endocrinology·D StanislausP M Conn
Apr 1, 1997·Current Opinion in Cell Biology·S M Mumby
Jun 15, 2007·Biochemistry·Yannick MarrariPhilip B Wedegaertner
Nov 29, 2007·Nature Reviews. Molecular Cell Biology·William M Oldham, Heidi E Hamm
Oct 3, 2002·Nature Reviews. Neuroscience·Alaa el-Din el-Husseini, David S Bredt
Dec 3, 2005·Hematology·Robert Flaumenhaft, Derek S Sim
Mar 14, 1998·Molecular Biology of the Cell·J MoralesH R Bourne
Sep 11, 2002·Molecular Biology of the Cell·David MichaelsonMark Philips
Jun 11, 2004·Annual Review of Biochemistry·Jessica E Smotrys, Maurine E Linder
Oct 5, 2007·Annual Review of Pharmacology and Toxicology·Hemal H PatelPaul A Insel
Mar 29, 2000·Biochemical and Biophysical Research Communications·K ScholichT B Patel
Feb 7, 2001·Biochemical and Biophysical Research Communications·F GuzziM Parenti
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·C Kleuss, A G Gilman
Jul 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·J H HurleyA P Fox
Oct 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Noriko MakitaTaroh Iiri
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·T IiriH R Bourne
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·C HuangS M Mumby
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·T IiriH R Bourne
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·S P SrinivasaM E Linder
Nov 11, 2006·Proceedings of the National Academy of Sciences of the United States of America·Gregory J DigbyNevin A Lambert
May 10, 2001·Proceedings of the National Academy of Sciences of the United States of America·P RondardH R Bourne
Dec 31, 2016·Biomolecules & Therapeutics·Xiaohan Zhang, Kyeong-Man Kim
Jun 5, 2004·FEBS Letters·Satoshi Takida, Philip B Wedegaertner
Jun 13, 2006·Seminars in Cell & Developmental Biology·Caroline NunnPaul R Albert
May 22, 2008·Electrophoresis·Berta SunyerGert Lubec
May 14, 2004·Journal of Neurochemistry·Hazem Hallak, Raphael Rubin
Jan 27, 2017·American Journal of Physiology. Cell Physiology·Anna R BusijaPaul A Insel
Feb 10, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ulrike MühlhäuserThomas Eschenhagen
Jun 23, 1998·The Journal of Biological Chemistry·J A Duncan, A G Gilman
May 20, 2000·The Journal of Biological Chemistry·C A Chen, D R Manning
May 10, 2000·The Journal of Biological Chemistry·T L BakerJ E Buss
May 31, 2020·Cancer Metastasis Reviews·Pascal Bernatchez
Jun 22, 1999·The Journal of Biological Chemistry·C S FishburnH R Bourne

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