PMID: 8966229Feb 1, 1996Paper

Reciprocal translocation frequency in irradiated sensitive and resistant human tumor cells in correlation with clonogenic in vitro cell survival: a possibility of tumor radiosensitivity prediction?

Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology
M Rave-FränkH P Rodemann

Abstract

We have investigated the yields of radiation-induced translocations in several human tumor cell lines and in normal diploid human fibroblasts by fluorescence in situ hybridization. The translocation yields were determined with respect to chromosome no. 1 in all cell lines investigated, and moreover in chromosomes nos. 2, 4 and 9 in fibroblasts and one tumor cell line. The chromosomes were "painted' with fluorescent whole chromosome-hybridization probes. The clonogenic survival of the studied cell lines was determined by standard colony-formation assay. We observed a higher frequency of reciprocal translocations in the radiosensitive cells MCF-7 and MDA-MB-436 as compared with the radioresistant cells CaSki and normal skin fibroblasts. Thus, the results suggest a possibility to predict the intrinsic tumor radiosensitivity on the basis of reciprocal translocation yield determined in cells irradiated in vitro. The correlation was observed in spite of the trisomy no. 1 which occurred in all three investigated tumor cell lines. On the other hand, the results obtained with different chromosomes in MCF-7 cells suggest that only chromosomes with relatively low "spontaneous' translocation yields are suitable for this kind of analysis.

References

Mar 1, 1990·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·L J Peters
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D PinkelJ Gray
May 1, 1993·Mutation Research·A T NatarajanM Z Zdzienicka

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Citations

Aug 1, 1996·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·P Virsik-PeuckertH Schmidberger
Nov 1, 1996·Mutation Research·A A EdwardsP Bryant
May 29, 1999·International Journal of Radiation Oncology, Biology, Physics·A GaussenC Parmentier
Nov 19, 2010·Radiation and Environmental Biophysics·Hendrik A WolffHans Christiansen
Nov 12, 1998·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·J DunstE Gebhart
Nov 30, 2018·The Journal of Cell Biology·Li QiangMark A McNiven

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