PMID: 2993319Sep 1, 1985

Reciprocal transmembranous receptor-cytoskeleton interactions in concanavalin A-activated platelets

The Journal of Cell Biology
M E WheelerR C Carroll

Abstract

Concanavalin A (Con A) has been used to activate platelets, inducing a specific interaction between the glycoprotein IIb-IIIa complex and the cytoskeleton of the activated platelet. In agreement with this, we have shown that Con A activates human platelets, initiating phosphorylation, secretion, and cytoskeletal formation. Con A and cytochalasin B were used to demonstrate a reciprocal interaction of the glycoprotein complex with the platelet cytoskeleton. Additionally, we have shown that a similar reciprocity is provided by the multivalent fibrin-fibrinogen platelet interaction found in the thrombin-induced clot. Con A differs from other activators in precipitating an apparent cytoskeletal core despite a complete inhibition of platelet activation by prostaglandin E1. We suggest, from this result, that Con A may be cross-linking a membrane-associated cytoskeletal complex present in the unactivated platelet.

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Citations

Aug 13, 2004·Journal of Neurobiology·Markus K KloseR Meldrum Robertson
Jan 1, 1995·Journal of Cellular Biochemistry·M TortiC Balduini
Mar 1, 1992·Cell Biochemistry and Function·M TortiF Sinigaglia

Related Concepts

Brevin
Isoactin
Caverject
Blood Platelets
Carrier Proteins
Concanavalin A
Microtrabecular Lattice
Gamma-Fibrinogen
Neoglycoproteins
Cell Surface Proteins

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