Reclassification of Paenibacillus larvae subsp. pulvifaciens and Paenibacillus larvae subsp. larvae as Paenibacillus larvae without subspecies differentiation

International Journal of Systematic and Evolutionary Microbiology
Elke GenerschIngemar Fries

Abstract

A polyphasic taxonomic study of the two subspecies of Paenibacillus larvae, Paenibacillus larvae subsp. larvae and Paenibacillus larvae subsp. pulvifaciens, supported the reclassification of the subspecies into one species, Paenibacillus larvae, without subspecies separation. Our conclusions are based on the analysis of six reference strains of P. larvae subsp. pulvifaciens and three reference strains and 44 field isolates of P. larvae. subsp. larvae. The latter originated from brood or honey of clinically diseased honey bee colonies or from honey of both clinically diseased and asymptomatic colonies from Sweden, Finland and Germany. Colony and spore morphology, as well as the metabolism of mannitol and salicin, did not allow a clear identification of the two subspecies and SDS-PAGE of whole-cell proteins did not support the subspecies differentiation. For genomic fingerprinting, repetitive element-PCR fingerprinting using ERIC primers and PFGE of bacterial DNA were performed. The latter method is a high-resolution DNA fingerprinting method proven to be superior to most other methods for biochemical and molecular typing and has not previously been used to characterize P. larvae. ERIC-PCR identified four different genotypes, whi...Continue Reading

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Citations

Apr 20, 2010·Applied Microbiology and Biotechnology·Elke Genersch
Aug 20, 2011·Current Microbiology·Angela Di PintoGiuseppina Tantillo
Sep 7, 2013·Genome Announcements·Ana OliveiraJoana Azeredo
Nov 16, 2013·Genome Announcements·Michael A ShefloSandra H Burnett
Dec 25, 2012·Journal of Bacteriology·Israel AlvaradoErnesto Abel-Santos
Mar 1, 2014·International Journal of Medical Sciences·Pattaraporn BoonsaiChanpen Chanchao
May 3, 2014·Proceedings. Biological Sciences·Javier Hernández LópezUlrike Riessberger-Gallé
Jul 2, 2015·Journal of Microbiological Methods·Israel AlvaradoHelen J Wing
Mar 3, 2012·Journal of Invertebrate Pathology·Karina AntúnezPablo Zunino
Mar 6, 2012·Journal of Invertebrate Pathology·Cristina Manuela MihaiHelge Schlüns
Feb 5, 2010·Journal of Invertebrate Pathology·Eva Forsgren
Nov 17, 2009·Journal of Invertebrate Pathology·Elke Genersch
Nov 17, 2009·Journal of Invertebrate Pathology·Jay D Evans, Marla Spivak
Sep 29, 2009·Veterinary Microbiology·Karina AntúnezPablo Zunino
Oct 4, 2008·Journal of Invertebrate Pathology·Igor LoncaricRudolf Moosbeckhofer
May 30, 2008·Veterinary Microbiology·Karina AntúnezPablo Zunino
Dec 7, 2007·Journal of Invertebrate Pathology·Esther Margarida A F BastosMarla Spivak
May 23, 2007·Veterinary Microbiology·K AntúnezP Zunino
Nov 7, 2006·Letters in Applied Microbiology·D C de GraafW Ritter
Oct 31, 2006·Insect Molecular Biology·X QinG M Weinstock
Apr 1, 2012·Environmental Microbiology Reports·Anne Fünfhaus, Elke Genersch
Jan 27, 2012·Evolution; International Journal of Organic Evolution·Robert M Brucker, Seth R Bordenstein
Jun 16, 2010·Environmental Microbiology·Dieter BehrensRobin F A Moritz

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