Recognition and specific degradation of bacteriophage T4 mRNAs.

Genetics
H Ueno, T Yonesaki

Abstract

Gene 61.5 of bacteriophage T4 has a unique role in gene expression. When this gene is mutated, mRNAs of many late genes are rapidly degraded, resulting in late-gene silencing. Here, we characterize an extragenic suppressor, ssf5, of a gene 61.5 mutation. ssf5 was found to be an amber mutation in motA, which encodes a transcription activator for T4 middle genes. When this gene is mutated, both degradation and specific cleavage of late-gene mRNA is induced after a delay, as exemplified by soc mRNA. Consequently, partial late-gene expression occurs. In an ssf5 genetic background, a gene 61.5 mutation exhibits a novel phenotype: in contrast to late-gene mRNA, middle-gene mRNA is stabilized and the expression of middle genes is prolonged. This is attributable to an activity of gene 61.5 specific for degradation of middle-gene mRNA. The degradation of middle-gene mRNA in the presence of a normal gene 61.5 appears in parallel with the degradation of late-gene mRNA in its absence. This observation suggests that the mRNA-degrading activity that silences late genes in cells infected with a gene 61.5 mutant is targeted to middle-gene mRNA when gene 61.5 is wild type. These results and the results obtained in the presence of a normal motA ...Continue Reading

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Citations

Aug 31, 2002·Virology·Kimberly LukeYakov Kogan
Sep 27, 2007·Genes & Genetic Systems·Yuichi OtsukaTetsuro Yonesaki
Jul 16, 2005·Genetics·Haruyo Yamanishi, Tetsuro Yonesaki
Oct 13, 2004·Virology·Hiroyuki Ueno, Tetsuro Yonesaki
Nov 7, 2002·Genes & Genetic Systems·Tetsuro Yonesaki
Nov 21, 2002·Genes & Genetic Systems·Hiroyuki Ueno, Tetsuro Yonesaki
Jan 29, 2005·Genetics·Yuichi Otsuka, Tetsuro Yonesaki
Nov 15, 2005·Genes & Genetic Systems·Takuma KanesakiTetsuro Yonesaki
Jan 21, 2003·Journal of Bacteriology·Yuichi OtsukaTetsuro Yonesaki
Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Eric S MillerWolfgang Rüger
Jan 24, 2002·Genetics·Toshie Kai, Tetsuro Yonesaki
Apr 28, 2010·Genetics·Yuichi OtsukaTetsuro Yonesaki
Oct 29, 2010·Genetics·Mitsunori KogaTetsuro Yonesaki

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