PMID: 2110821Mar 27, 1990Paper

Recognition between mitomycin C and specific DNA sequences for cross-link formation

Biochemistry
H Borowy-BorowskiM Tomasz

Abstract

An extensive series of oligodeoxyribonucleotides was reacted with reductively activated mitomycin C (MC), and the resulting cross-linked drug-oligonucleotide complexes were isolated by reverse-phase HPLC and characterized by nucleoside and MC-nucleoside adduct analysis. HPLC also served for assay of the yield of cross-linked oligonucleotides. AT-rich duplex oligonucleotides, containing a single central CG.CG, gave high yields of cross-links between the two guanines while those having GC.GC, instead, gave none. In another series, the central sequences CGC.GCG and CGC.ICG both yielded 50% cross-link while CGC.GCI was completely resistant. Cross-linking was conducted also in two steps: Oligonucleotides substituted monofunctionally by MC at guanine at either a CG or GC sequence were annealed with their complementary strands followed by reductive reactivation of the bound MC to form a cross-link. The CG oligomers were cross-linked quantitatively while the GC ones were again resistant. These results show unambiguously that the MC cross-link is absolutely specific to the CG.CG duplex sequence, confirming our previous finding [Chawla, A.K., Lipman, R., & Tomasz, M. (1987) in Structure and Expression, Volume 2: DNA and Its Drug Complexe...Continue Reading

References

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Citations

May 1, 1994·Molecular Carcinogenesis·N S SrikanthH L Gurtoo
Aug 17, 1996·Mutation Research·P D Lawley, D H Phillips
Sep 18, 1998·Mutation Research·M Buchwald, E Moustacchi
May 17, 2001·Bioorganic & Medicinal Chemistry·V S LiH Kohn
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·B ReissJ Schell
Jun 25, 1995·Nucleic Acids Research·R J CainG D Glick
Jul 1, 2008·Journal of the American Chemical Society·Elise ChampeilMaria Tomasz
Feb 20, 2016·Bioorganic Chemistry·Elise ChampeilAnne-Marie Sapse
Nov 15, 2012·Mass Spectrometry Reviews·Catherine Silvestri, Jennifer S Brodbelt
Mar 18, 1995·Chemico-biological Interactions·B Jollès, A Laigle
Apr 22, 2006·Experimental Cell Research·H I Abdel-HalimJ J W A Boei
Feb 2, 1999·Toxicology and Applied Pharmacology·R A CoulombeF R Stermitz
Aug 27, 2013·Critical Reviews in Oncology/hematology·Stéphane PuyoPhilippe Pourquier
Sep 1, 1995·Chemistry & Biology·M Tomasz
Nov 30, 1993·Biochemistry·J T Millard, T M Beachy
Mar 6, 2018·Chemistry : a European Journal·William AguilarElise Champeil
Nov 1, 2018·Molecular Microbiology·Peter E Burby, Lyle A Simmons
Apr 23, 2008·Current Protocols in Nucleic Acid Chemistry·Gary D Glick
Feb 17, 1998·Environmental and Molecular Mutagenesis·A J WarrenJ W Hamilton
Nov 14, 2015·G3 : Genes - Genomes - Genetics·Annie S TamAnn M Rose
Oct 26, 2016·International Journal of Oncology·Shu-Yuan ChengElise Champeil

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