Recognition events in AM symbiosis: analysis of fungal gene expression at the early appressorium stage

Fungal Genetics and Biology : FG & B
Magadalene Breuninger, Natalia Requena

Abstract

Arbuscular mycorrhizal symbiosis is induced upon a series of recognition events involving the reorganization of both plant and fungal cellular programs culminating in the formation of appressoria on the epidermal root cells. In this work we monitored for the first time the genetic changes occurring in the fungal partner during early appressorium development. We established an in vitro system of Glomus mosseae and Petroselinum crispum for studying appressorium formation and found that after 120 h first appressoria developed in the root epidermis. We have constructed a fungal subtractive suppressive library enriched in genes up-regulated at this stage. Our aim was to identify early signaling events during plant recognition leading to appressoria formation. The library contains 375 clones with an average size of 500 bp. From these, 200 clones were sequenced and most of them represent gene fragments with no known homologues (63%) and therefore putative new genes specific to the mycorrhiza symbiosis. Reverse-Northern blot and RT-PCR analyses confirmed that ca. 30% of the genes present in the library were up-regulated upon plant induction after 120 h. Among the genes with homologues in other organisms we found several genes common to...Continue Reading

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Citations

Sep 28, 2006·Mycorrhiza·Raffaella Balestrini, Luisa Lanfranco
Sep 1, 2006·Molecular Plant-microbe Interactions : MPMI·Nardjis AmiourEliane Dumas-Gaudot
Sep 13, 2005·Annual Review of Microbiology·Maria J Harrison
Feb 16, 2008·The New Phytologist·Francis Martin
Sep 2, 2006·The New Phytologist·Uta Paszkowski
May 20, 2015·World Journal of Microbiology & Biotechnology·Sunayna GahlotNarendra Tuteja
Feb 28, 2009·Molecular Plant-microbe Interactions : MPMI·Pascale M A SeddasVivienne Gianinazzi-Pearson
May 17, 2007·The New Phytologist·Aurora OcónNatalia Requena
Oct 2, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Pedro T LimaElio Sucena
Apr 1, 2006·Annals of Botany·Kohki Akiyama, Hideo Hayashi

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