Recognition imaging with a DNA aptamer

Biophysical Journal
Liyun LinStuart M Lindsay

Abstract

We have used a DNA-aptamer tethered to an atomic force microscope probe to carry out recognition imaging of IgE molecules attached to a mica substrate. The recognition was efficient (approximately 90%) and specific, being blocked by injection of IgE molecules in solution, and not being interfered with by high concentrations of a second protein. The signal/noise ratio of the recognition signal was better than that obtained with antibodies, despite the fact that the average force required to break the aptamer-protein bonds was somewhat smaller.

References

Aug 30, 1990·Nature·A D Ellington, J W Szostak
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·P HinterdorferH Schindler
Dec 24, 2002·Biophysical Journal·Hongda WangStuart M Lindsay
Aug 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·C StrohP Hinterdorfer
Sep 4, 2004·Biophysical Journal·Cordula M StrohPeter Hinterdorfer

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Citations

Nov 8, 2007·Journal of the American Chemical Society·Liyun LinJohn C Chaput
May 14, 2008·Human Gene Therapy·Claudia M DollinsBruce A Sullenger
Dec 4, 2009·Nucleic Acids Research·Yusuke MiyachiAkihiko Kondo
Dec 19, 2008·Epigenetics & Chromatin·Hongda WangStuart Lindsay
May 9, 2014·Expert Opinion on Drug Delivery·Constanze LamprechtAndreas Ebner
Aug 9, 2011·Journal of Bioscience and Bioengineering·Yusuke MiyachiAkihiko Kondo
Mar 5, 2016·Accounts of Chemical Research·Subhadip Senapati, Stuart Lindsay
Feb 1, 2012·Journal of Molecular Recognition : JMR·Dionne C G KleinBjørn T Stokke
Dec 31, 2010·Biophysical Journal·Parminder KaurStuart Lindsay
Sep 16, 2009·Biophysical Journal·Liyun LinStuart Lindsay
Mar 5, 2014·Nucleosides, Nucleotides & Nucleic Acids·Yusuke MiyachiAkihiko Kondo
Mar 1, 2018·Annual Review of Analytical Chemistry·Anisha N Patel, Christine Kranz
May 9, 2007·Nanotechnology·Jason ReedJames K Gimzewski
Oct 24, 2014·Nanotechnology·Congzhou Wang, Vamsi K Yadavalli
Jun 6, 2009·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Rong ZhuPeter Hinterdorfer
Jun 19, 2008·Journal of the American Chemical Society·Maxim V BerezovskiSergey N Krylov
Oct 8, 2009·Nano Letters·Junguang JiangHongda Wang
Apr 12, 2012·The Journal of Physical Chemistry. B·Bin WangBingqian Xu
May 6, 2011·Bioconjugate Chemistry·Linda WildlingHermann J Gruber

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Proceedings of the National Academy of Sciences of the United States of America
C StrohPeter Hinterdorfer
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