Recognition motif and mechanism of ripening inhibitory peptides in plant hormone receptor ETR1

Scientific Reports
Dalibor MilićGeorg Groth

Abstract

Synthetic peptides derived from ethylene-insensitive protein 2 (EIN2), a central regulator of ethylene signalling, were recently shown to delay fruit ripening by interrupting protein-protein interactions in the ethylene signalling pathway. Here, we show that the inhibitory peptide NOP-1 binds to the GAF domain of ETR1 - the prototype of the plant ethylene receptor family. Site-directed mutagenesis and computational studies reveal the peptide interaction site and a plausible molecular mechanism for the ripening inhibition.

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Citations

Apr 26, 2020·The Journal of Biological Chemistry·Brad M Binder
Jan 31, 2021·Biophysical Journal·Christopher PflegerHolger Gohlke
Feb 9, 2021·Computational and Structural Biotechnology Journal·Francisca ContrerasUlrich Schwaneberg
Jan 20, 2021·Journal of Chemical Information and Modeling·Daniel MulnaesHolger Gohlke
May 6, 2021·Journal of Chemical Information and Modeling·Christina NutschelHolger Gohlke
Jun 19, 2021·Annual Review of Plant Biology·Xuan Lan Thi HoangLam-Son Phan Tran
Dec 18, 2020·ACS Medicinal Chemistry Letters·Andrea DorstKarl Gademann

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Methods Mentioned

BETA
microscale thermophoresis
PCR
circular dichroism
peptide folding

Software Mentioned

TopDock
FIRST
DrugScore
DISOPRED
K2D2
HADDOCK
SciPy
FIRST ( Floppy Inclusions and Rigid Substructure Topography )
CDSSTR
GraphPad

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