Recognition of abasic sites and single base bulges in DNA by a metalloinsertor.

Biochemistry
Brian M ZeglisJacqueline K Barton

Abstract

Abasic sites and single base bulges are thermodynamically destabilizing DNA defects that can lead to cancerous transformations if left unrepaired by the cell. Here we discuss the binding properties with abasic sites and single base bulges of Rh(bpy)(2)(chrysi)(3+), a complex previously shown to bind thermodynamically destabilized mismatch sites via metalloinsertion. Photocleavage experiments show that Rh(bpy)(2)(chrysi)(3+) selectively binds abasic sites with affinities of 1-4 x 10(6) M(-1); specific binding is independent of unpaired base identity but is somewhat contingent on sequence context. Single base bulges are also selectively bound and cleaved, but in this case, the association constants are significantly lower ( approximately 10(5) M(-1)), and the binding is dependent on both unpaired base identity and bulge sequence context. A wide variety of evidence, including strand scission asymmetry, binding enantiospecificity, and MALDI-TOF cleavage fragment analysis, suggests that Rh(bpy)(2)(chrysi)(3+) binds abasic sites, like mismatches, through insertion of the bulky chrysi ligand into the base pair stack from the minor groove side and ejection of the unpaired base. At single base bulge sites, a similar, though not identica...Continue Reading

References

May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G VesnaverK J Breslauer
Aug 15, 1986·Science·J K Barton
Sep 29, 1995·The Journal of Biological Chemistry·I GoljerP H Bolton
Oct 1, 1995·Trends in Biochemical Sciences·R D Kolodner
May 20, 1998·Cancer·I I ArzimanoglouH R Barber
Jul 18, 2000·Biopolymers·J LhommeM Demeunynck
Mar 29, 2001·Trends in Genetics : TIG·L J Marnett, J P Plastaras
Dec 26, 2001·Chemical Reviews·K E ErkkilaJ K Barton
Mar 20, 2002·Biochimie·Sandrine Jacob, Françoise Praz
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Lawrence A LoebJon P Anderson
Mar 1, 2003·Proceedings of the National Academy of Sciences of the United States of America·Henrik JunickeJacqueline K Barton
Apr 18, 2003·Frontiers in Bioscience : a Journal and Virtual Library·Guo-Min Li
Jun 5, 2004·Bioorganic & Medicinal Chemistry Letters·Akio KoboriKazuhiko Nakatani
Feb 9, 2006·Chemical Reviews·Ravi R IyerPaul L Modrich
May 25, 2006·Journal of the American Chemical Society·Jens Brunner, Jacqueline K Barton
Jun 29, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Joy L MorganF Richard Keene
Nov 23, 2006·DNA Repair·David M Wilson, Vilhelm A Bohr
Dec 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Valérie C PierreJacqueline K Barton
Apr 5, 2007·Nature Protocols·Brian M Zeglis, Jacqueline K Barton
Jun 22, 2007·Nature·Sheila S DavidSucharita Kundu
Sep 20, 2007·Journal of the American Chemical Society·Christine CordierJacqueline K Barton
Nov 9, 2007·Chemical Communications : Chem Comm·Brian M ZeglisJacqueline K Barton
May 22, 2008·Journal of the American Chemical Society·Brian M ZeglisJacqueline K Barton
May 3, 1999·Angewandte Chemie·Chien-Chung ChengWen Jwu Wang

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Citations

Feb 1, 2012·Inorganic Chemistry·Attilio V Vargiu, Alessandra Magistrato
Apr 18, 2012·Inorganic Chemistry·Imee Marie A del MundoJanet R Morrow
May 21, 2009·Inorganic Chemistry·Mi Hee LimJacqueline K Barton
Dec 1, 2009·Nucleic Acids Research·Conceição A S A MinettiKenneth J Breslauer
Feb 9, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Fangfei LiF Richard Keene
Nov 25, 2011·Journal of Inorganic Biochemistry·Kamalakannan PalanichamyAllyn C Ontko
Sep 18, 2015·Chemical Communications : Chem Comm·Naoko KoteraMarie-Paule Teulade-Fichou
Jan 18, 2012·Chembiochem : a European Journal of Chemical Biology·Kevin E NelsonYi Lu
Nov 26, 2009·Chemistry : a European Journal·Anton GranzhanMarie-Paule Teulade-Fichou
Apr 14, 2016·Journal of the American Chemical Society·Adam N BoyntonJacqueline K Barton
May 2, 2015·Organic & Biomolecular Chemistry·Shyam K KondaJ Grant Collins
Nov 12, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Jian PengHua Liu
Dec 19, 2014·Dalton Transactions : an International Journal of Inorganic Chemistry·Kevin E SitersJanet R Morrow
Jun 29, 2017·Inorganic Chemistry·Adam N BoyntonJacqueline K Barton
Jan 8, 2019·Chemistry : a European Journal·Kelsey M BoyleJacqueline K Barton
Aug 29, 2019·Dalton Transactions : an International Journal of Inorganic Chemistry·David Paul Elisa DayanidhiVaidyanathan Vaidyanathan Ganesan
Aug 23, 2019·Journal of Biomolecular Structure & Dynamics·Young-Ae LeeSeog K Kim
Nov 26, 2010·Inorganic Chemistry·Brigitte R SpencerJeffrey M Zaleski
Nov 15, 2017·Journal of the American Chemical Society·Adela NanoJacqueline K Barton

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