PMID: 8974448Sep 1, 1995Paper

Recognition of alternating oligopurine/oligopyrimidine tracts of DNA by oligonucleotides with base-to-base linkages

Bioconjugate Chemistry
B W ZhouC Hélène

Abstract

A new concept is presented to design and synthesize modified oligonucleotides in order to extend the range of double-helical DNA sequences that can be recognized by oligonucleotides via triple helix formation. The DNA target is composed of adjacent oligopurine.oligopyrimidine domains where the oligopurine sequences alternate on the two DNA strands. Canonical (C,T)-motif triple helices are formed with each oligopurine.oligopyrimidine domain of the target sequence. The two third-strand oligonucleotides were joined together via an appropriate linker between the two terminal bases with either a 3'-3' or a 5'-5' polarity. Molecular modeling was used to predict the optimal length of the linker bridging two terminal bases. The interaction of DNA with such a modified oligonucleotide containing a C3'-3'U linkage was studied by thermal dissociation, footprinting, and gel retardation experiments. They provide experimental evidence that the oligonucleotide does form a switched triple helix on this extended DNA target sequence. The binding of the so-called "switch oligonucleotide" is enhanced as compared to the two unlinked parental oligonucleotides which form triple helices with each oligopurine.oligopyrimidine domain of the target sequence.

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C GiovannangéliC Hélène
May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·K YoonA L Weis
Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G Duval-ValentinC Hélène
Aug 1, 1965·Journal of Molecular Biology·C R Cantor, I Tinoco

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Citations

Jun 1, 1996·Current Opinion in Structural Biology·J S SunC Hélène
Sep 21, 2001·Nucleosides, Nucleotides & Nucleic Acids·S P ParelC J Leumann
Aug 1, 1997·Antisense & Nucleic Acid Drug Development·C Giovannangeli, C Hélène
Jan 4, 2003·Chemical Communications : Chem Comm·Kazuo ShinozukaHiroaki Sawai

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