PMID: 2116484Aug 1, 1990Paper

Recognition of keratinocytes by cytotoxic T cells specific for conventional HLA class-I alloantigen

The Journal of Investigative Dermatology
F W Symington, E B Santos

Abstract

This study analyzed whether human keratinocytes (KC) express conventional HLA class-I molecules as detected by class-I-specific cytotoxic T lymphocytes (CTL), and whether exposure of KC to interferon-gamma (IFN-g) is required for CTL recognition. Basal KC grown in serum-free medium and exposed to recombinant IFN-g for 24-96 h were used as targets in 51Cr-release assays. Target-cell susceptibilities to lysis were compared by analyzing the lytic unit (LU) activity of a given CTL population against IFN-g-treated and untreated KC. CTL effectors were cloned from alloantigen-primed cultures by limiting dilution in the presence of antigenic B lymphoblastoid cells (BCLL) and IL-2. These T-cell clones lysed appropriate BCLL and PHA blasts but not third-party BCLL or K562. Lysis of antigenic BCLL was specifically blocked by antibodies against CD3 or class-I antigens. Specificity of the clones for conventional class-I antigen was demonstrated by cytotoxicity tests employing a panel of HLA-typed BCLL. The clones specifically lysed KC syngeneic with the original effector immunogen, and lysis was also blocked by anti-class-I antibodies. The effect of IFN-g treatment was to increase KC susceptibility to lysis by these clones. From 3-25 times ...Continue Reading

Citations

Mar 10, 2001·Clinical and Diagnostic Laboratory Immunology·M ScottA B Moscicki
Jul 10, 1999·Immunological Reviews·I H FrazerM Sharkey
Jul 16, 2009·Experimental Dermatology·Fang ZhouGraham R Leggatt

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