PMID: 2410776Jun 1, 1985Paper

Recognition of peptide orientation: studies with angiotensin II in the guinea-pig

Molecular Immunology
J NussbergerE Haber

Abstract

Antipeptide sera of defined subspecificities were obtained when angiotensin II was unidirectionally conjugated to its carrier protein. Antisera with predominantly amino terminal specificity were produced when guinea-pigs were immunized with angiotensin II conjugated via its carboxy terminus to thyroglobulin. When angiotensin II was coupled via its amino terminus, carboxy terminal-specific antisera were obtained. The same conjugates failed to provide antisera with the corresponding specificities when rabbits were used instead of guinea-pigs. This work demonstrates that the peptide-protein coupling strategy may affect the specificity of the resulting antiserum.

References

Jun 1, 1969·The Journal of Clinical Endocrinology and Metabolism·G E Abraham
Nov 1, 1984·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·J NussbergerE Haber
Jan 1, 1983·Journal of Immunological Methods·J NussbergerE Haber
Jan 1, 1983·Histochemistry·E Hackenthal, R Taugner

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