Recognition of Pneumocystis carinii antigens by local antibody-secreting cells following resolution of P. carinii pneumonia in mice

The Journal of Infectious Diseases
Francis GigliottiAllen G Harmsen

Abstract

To examine the repertoire of Pneumocystis carinii antigens recognized by antibody-secreting B cells from tracheobronchial lymph nodes isolated immediately following recovery from P. carinii pneumonia, monoclonal antibodies (MAbs) were produced from these cells. In contrast to previous studies of systemic immunity, P. carinii gpA was not the immunodominant antigen recognized by these B cells. Forty-nine (91%) of 54 P. carinii-specific hybridoma culture supernatants reacted with P. carinii antigens other than gpA. Many of the resulting MAbs recognized a previously uncharacterized antigen expressed on the surface of both cysts and trophozoites. Western blotting using one of the cloned MAbs revealed reactivity with a broad range of antigenic material, with the most intense reactivity in the 50- to 65-kDa region of the blot. The antigens identified by these MAbs merit further investigation regarding protective immunity to P. carinii because they were recognized by B cells in the context of recovery from P. carinii pneumonia.

Citations

Nov 19, 2008·The Journal of Infectious Diseases·Heather M KlingKaren A Norris
Apr 12, 2012·Clinical Microbiology Reviews·Alison Morris, Karen A Norris
Feb 28, 2003·American Journal of Respiratory Cell and Molecular Biology·Ian J MoralesCharles F Thomas
Mar 15, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·S L VargasF Gigliotti
Sep 5, 2013·The Journal of Eukaryotic Microbiology·Peter D Walzer
May 7, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·K DalyP D Walzer
Aug 16, 2003·Blood·Brian J RybarczykPatricia J Simpson-Haidaris
Apr 21, 1999·Molecular Microbiology·E B LugliA E Wakefield
Jan 23, 1999·Infection and Immunity·J M PascaleG L McLaughlin
Mar 6, 1999·Clinical and Diagnostic Laboratory Immunology·P D Walzer

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