PMID: 6976406Dec 1, 1981Paper

Recognition of polymorphic H-2 domains by T lymphocytes. I. Functional role of different H-2 domains for the generation of alloreactive cytotoxic T lymphocytes and determination of precursor frequencies

The Journal of Experimental Medicine
C WeyandG J Hämmerling

Abstract

In the present communication, the repertoire of alloreactive cytotoxic T lymphocytes (CTL) clones was quantitatively investigated by limiting dilution analysis and by target inhibition with a panel of monoclonal antibodies (mAb). These mAb have previously been shown to define two distinct alloantigenic domains, A and B, on the H-2Kk molecule. The Poisson distribution analysis of H-2Kk-specific CTL clones generated in a limiting dilution system revealed three CTL populations with different precursor frequencies. The high frequent population is suppressed by an unknown suppressive mechanism that allows less frequent CTL populations to become visible. Target inhibition studies with a panel of Kk-specific mAb showed that these CTL populations differ not only in their precursor frequency but also in their specificity for different H-2 epitopes on the Kk molecule. Thus clones of the high frequency population are almost exclusively specific for determinants within domain A. In contrast, the low frequency population displays predominant specificity for determinants of domain B, while the population with medium frequency is blocked equally well by mAb against either domains A or B. Each mAb blocked only a fraction of clones indicating t...Continue Reading

References

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Citations

Jan 1, 1983·Springer Seminars in Immunopathology·K EichmannH U Weltzien
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G J HämmerlingU Hämmerling
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·K OzatoJ G Seidman
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·B Stockinger
Jun 13, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anne MustersNiek de Vries
Sep 1, 1983·European Journal of Immunology·N ChristensenJ Marbrook
Nov 1, 1982·European Journal of Immunology·N KochU Hämmerling
Apr 1, 1989·European Journal of Immunology·G J HämmerlingJ Moreno
Jul 1, 1986·European Journal of Immunology·U HorstmannG J Hämmerling
Jan 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·E J SuchinL A Turka

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