PMID: 9188627Jul 1, 1997Paper

Recognition of prominent viral epitopes induced by immunization with human immunodeficiency virus type 1 regulatory genes

Journal of Virology
J HinkulaB Wahren

Abstract

Mice immunized with the regulatory genes nef, rev, and tat from human immunodeficiency virus type 1 developed both humoral and cellular immune responses to the gene products Nef, Rev, and Tat. This study demonstrates that it is feasible to induce immune reactions to all of these regulatory gene products. Humoral responses were seen after DNA boosts, while potent T-cell proliferative responses were noted already after a single immunization. A Th1-directed immune response was demonstrated early after immunization. A 3- to 75-fold-stronger T-cell response was seen in animals receiving DNA epidermally compared to that in animals receiving intramuscular injections. Nef, Rev, and Tat putative B- and T-cell epitopes were clearly mapped by using peptides derived from the regulatory proteins and were similar to those which are detected in human immunodeficiency virus infection. Although immunization by the Nef, Rev, and Tat proteins raised high immunoglobulin G titers in serum, the epitope spreading appeared broader after DNA immunization. The combination of all of these regulatory genes together with two genes for structural proteins, the envelope and gag genes, demonstrated that a combined approach is feasible in that reactivities to ...Continue Reading

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Citations

Apr 21, 2004·AIDS·Lars H LundJorma Hinkula
Aug 24, 2002·Hybridoma and Hybridomics·Watchara KasinrerkKriangkrai Chawansuntati
Oct 9, 2009·International Reviews of Immunology·Antonella CaputoBarbara Ensoli
May 1, 1999·Biological Chemistry·Y AsakuraJ Hinkula
May 25, 1999·Microbiology and Immunology·S SasakiK Okuda
Jun 25, 1999·Acta Oncologica·B Wahren
May 21, 2005·Reviews in Medical Virology·J RajcániI Rezuchová
Oct 25, 2002·DNA and Cell Biology·Emanuele Fanales-BelasioBarbara Ensoli
Jun 17, 2009·Expert Opinion on Biological Therapy·Matti SällbergOla Weiland
Oct 14, 2020·Current HIV Research·Maryam-Sadat Yadavar-NikraveshAzam Bolhassani

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