Recognition of Schistosoma mansoni cathepsins B and L by human IgG1 and IgG4 antibodies

Parasite Immunology
J GroganM Klinkert

Abstract

Human schistosome infections are chronic in nature and elevated IgG4 levels are associated with length of exposure. To examine how structure, localization and dynamics of exposure of a protein to the immune system can affect antibody isotype expression, specific antibody levels against two Schistosoma mansoni recombinant cathepsin molecules were determined in S. mansoni-infected individuals. Cathepsin B (rCatB, secreted in the gut) and cathepsin L (rCatL, localized in the reproductive structures) were used to determine IgG1 reactivity, as a measure of the stimulation of the immune response, and the switch to IgG4 as an indicator of the dynamics and rate of presentation to the immune system. Sera from three groups of S. mansoni-infected patients were used: individuals with life-long exposure in an endemic area in Burundi, a group from a recent endemic focus in Senegal, and of acutely infected European travellers. We report for the first time the ability of rCatL to trigger an immune response and show distinct antibody isotype reactivity between rCatB versus rCatL. Patients harbouring long-term chronic infections had elevated levels of IgG4 to both antigen, whereas individuals infected for less than four years had high IgG4 to rC...Continue Reading

Citations

May 3, 2002·Molecular and Biochemical Parasitology·Conor R CaffreyMohammed Sajid
Jul 10, 1998·Molecular and Biochemical Parasitology·A LoukasR M Maizels
Jun 20, 2002·Veterinary Parasitology·Jane L KinsellaMichael F Ryan
Mar 27, 2010·Cellular and Molecular Life Sciences : CMLS·Elissaveta B TchoubrievaBernd H Kalinna
Dec 15, 2015·Acta Tropica·Manaw SangfuangNarin Preyavichyapugdee
Jun 3, 2008·Vaccine·Jeffrey M BethonyAlex Loukas
Apr 24, 2004·Trends in Parasitology·Conor R CaffreyMohammed Sajid

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