Recognition of tRNA(Gly) by three widely diverged glycyl-tRNA synthetases

Journal of Molecular Biology
N NamekiT Hasegawa

Abstract

Glycyl-tRNA synthetase (GlyRS) is an unusual aminoacyl-tRNA synthetase because it varies in its quarternary structure between organisms; Escherichia coli GlyRS is an alpha2beta2 tetramer, whereas those of Thermus thermophilus and yeast are alpha2 dimers. In contrast, the tRNA(Gly) sequence is virtually identical in E. coli and T. thermophilus but very different in yeast. In this study, we examined the molecular recognition of tRNA(Gly) by three widely diverged GlyRSs using in vitro tRNA transcripts. Mutation studies showed that the discriminator base at position 73, the second base-pair, C2 x G71, in the acceptor stem, and the anticodon nucleotides, C35 and C36, contribute to the specific aminoacylation of all three GlyRSs, the discriminator base differing between prokaryotes (U73) and eukaryotes (A73). However, we found differences between yeast and two bacteria around the second base-pair in the acceptor stem. The first base-pair, G1 x C72, is important for glycylation in E. coli and T. thermophilus, whereas the third base-pair, G3 x C70, is important for glycylation in yeast. These findings indicate that despite such large differences of the two prokaryotic GlyRSs, tRNA(Gly) identity has been essentially conserved in prokary...Continue Reading

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Citations

Mar 2, 2012·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Aron Szenes, Gábor Pál
Apr 13, 1999·Nucleic Acids Research·S Brubacher-KauffmannA M Duchêne
Jun 2, 2005·Biochimie·Thalia SalinasAnne-Marie Duchêne
Nov 1, 2000·The Journal of Biological Chemistry·J D DignamJ B Chaires
Apr 22, 2015·BMC Evolutionary Biology·Gregory P FournierJohann Peter Gogarten
Mar 29, 2000·Biopolymers·P J Beuning, K Musier-Forsyth
Aug 21, 2015·Journal of Molecular Evolution·Koji Tamura
Mar 19, 2020·Mitochondrion·Jessica M Warren, Daniel B Sloan
Nov 5, 1999·Journal of Molecular Biology·A SomaH Himeno
Nov 19, 2008·Biochimie·Stamatina GiannouliConstantinos Stathopoulos

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