PMID: 8601612Apr 1, 1996Paper

Recognition of two classes of oligoproline sequences in profilin-mediated acceleration of actin-based Shigella motility

The Journal of Cell Biology
W L ZeileF S Southwick

Abstract

The gram negative rod Shigella flexneri uses it surface protein IcsA to induce host cell actin assembly and to achieve intracellular motility. Yet, the IcsA protein lacks the oligoproline sequences found in ActA, the surface protein required for locomotion of the gram positive rod Listeria monocytogenes. Microinjection of a peptide matching the second ActA oligoproline repeat (FEFPPPPTDE) stops Listeria locomotion (Southwick, F.S., and D.L. Purich. 1994a. Proc. Natl. Acad. Sci. USA. 91:5168-5172), and submicromolar concentrations (intracellular concentration 80-800 nM) similarly arrest Shigella rocket-tail assembly and intracellular motility. Coinjection of a binary solution containing profilin and the ActA analogue increased the observed rates of intracellular motility by a factor of three (mean velocity 0.90 +/- 0.07 mu m/s, SD n=16 before injection vs 0.3 +/- 0.1 mu m/s, n=33 postinjection, intracellular concentration = 80 nM profilin plus 80 nM ActA analogue). Recent evidence suggests the ActA analogue may act by displacing the profilin-binding protein VASP (Pistor, S.C., T. Chakaborty, V. Walter, and J. Wehland. 1995. Curr. Biol. 5:517-525). At considerably higher intracellular concentrations (10 muM), the VASP oligoprolin...Continue Reading

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Citations

Jan 1, 1997·Cell Motility and the Cytoskeleton·M B Goldberg
Apr 1, 2000·Cell Motility and the Cytoskeleton·F S SouthwickD L Purich
Sep 5, 2002·Cell Motility and the Cytoskeleton·Fangliang ZhangDaniel L Purich
Feb 1, 1997·Current Opinion in Cell Biology·S Higley, M Way
Jun 6, 2000·The Journal of Experimental Medicine·T SuzukiC Sasakawa
Apr 8, 2009·Infection and Immunity·Sarah E SzarowiczFrederick S Southwick
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·M B Goldberg
Jan 19, 1999·Annual Review of Cell and Developmental Biology·S Dramsi, P Cossart
Jan 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Laura LarsonFrederick Southwick
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·W L ZeileF S Southwick
Sep 23, 1997·The Journal of Cell Biology·R O LaineF S Southwick
Mar 11, 2004·Cellular Microbiology·Shabeen AllyMarcia B Goldberg
Apr 21, 2007·The EMBO Journal·Russell L DuringFrederick S Southwick
Oct 23, 2012·Cellular Microbiology·Ellen A BishaiFrederick S Southwick
Oct 30, 2001·International Journal of Medical Microbiology : IJMM·T Adam
Aug 18, 2001·Current Biology : CB·C J MerrifieldS E Moss
Dec 31, 1997·Biochimica Et Biophysica Acta·K SchlüterM Rothkegel
Jun 2, 1998·Biochimica Et Biophysica Acta·I LasaP Cossart
Jan 25, 2002·Biophysical Journal·Richard B Dickinson, Daniel L Purich
Nov 30, 1999·Molecular Cell Biology Research Communications : MCBRC·D L Purich, F S Southwick
Nov 13, 1999·Nature Cell Biology·C J MerrifieldW Almers
Aug 11, 2001·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·A D Rutenberg, M Grant
Nov 27, 2002·Cell Motility and the Cytoskeleton·Frederick S SouthwickDaniel L Purich
Jun 27, 2000·The Journal of Biological Chemistry·H MimuroC Sasakawa
Sep 28, 2017·Nature Communications·Dennis ZimmermannDavid R Kovar
Jan 12, 2005·The Journal of Biological Chemistry·Gurjit SidhuFrederick Southwick

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