Recognition of Z-RNA and Z-DNA determinants by polyamines in solution: experimental and theoretical studies.

Journal of Biomolecular Structure & Dynamics
H S BasuL J Marton

Abstract

Protonated polyamines are among the most efficient cations that induce the left-handed Z-form in certain polynucleotides. It is not known, however, whether these cations bind to specific sites on Z-sequences in solution. We have studied potential polyamine binding sites by measuring the effects of polyamines on the binding of purified immunoglobulins (IgGs) to different regions of the Z-helix and by molecular mechanics modeling. The specific binding of anti-Z-DNA and anti-Z-RNA IgGs to Z-helices was studied as a function of spermidine or spermine concentration. The effect of polyamines on the antibody-nucleic acid interaction was different for IgGs with different specificities for various determinants on the Z-helix. Polyamines inhibit the binding of certain anti-Z IgGs directed against specific sites probably at or near the interface between the major convex surface and the phosphate backbone, most likely by competing with the antibody binding site(s). In contrast, polyamines have no effect on other anti-Z IgGs directed against sites determined by the phosphate backbone. Furthermore, these cations can enhance the binding of anti-Z IgG directed against bulky groups at the C-5 position on the major convex surface of the helix; t...Continue Reading

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Citations

Sep 15, 2000·Bioorganic & Medicinal Chemistry Letters·I S BlagbroughS A David
Sep 1, 1993·Biopolymers·K G KrishnaM W Pandit
May 1, 1991·Journal of Cellular Biochemistry·B G FeuersteinL J Marton
Apr 28, 2007·Nature Reviews. Drug Discovery·Robert A Casero, Laurence J Marton
Oct 1, 2011·Organic & Biomolecular Chemistry·Lital ZilbershteinBilha Fischer
Mar 23, 2021·Biotechnology and Bioengineering·Hannah Panlilio, Charles V Rice
Oct 9, 2021·Signal Transduction and Targeted Therapy·Wenjuan MaYunfeng Lin

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