Recombinant β-defensin 126 promotes bull sperm binding to bovine oviductal epithelia

Reproduction, Fertility, and Development
A LyonsS Fair

Abstract

Primate β-defensin 126 regulates the ability of spermatozoa to bind to oviductal epithelial cells invitro. Bovine β-defensin 126 (BBD126) exhibits preferential expression in the cauda epididymis of the bull, but there have been few studies on its functional role in cattle. The aim of the present study was to examine the role of BBD126 in bull sperm binding to bovine oviductal epithelial cell (BOEC) explants. BBD126 has been shown to be highly resistant to the standard methods of dissociation used in other species and, as a result, corpus epididymal spermatozoa, which have not been exposed to the protein, were used to study the functional role of BBD126. Corpus epididymal spermatozoa were incubated with recombinant (r) BBD126 in the absence or presence of anti-BBD126 antibody. Addition of rBBD126 significantly enhanced the ability of epididymal spermatozoa to bind to BOEC explants (P<0.05). Anti-BBD126 antibody blocked the BBD126-mediated increase in sperm binding capacity. Ejaculated spermatozoa, which are coated with native BBD126 protein but also a large number of seminal plasma proteins invivo, were incubated with rBBD126 in the absence or presence of the anti-BBD126 antibody. Addition of rBBD126 significantly enhanced the a...Continue Reading

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Citations

May 22, 2018·Animal : an International Journal of Animal Bioscience·S Fair, P Lonergan
Oct 28, 2019·Andrologia·Reyhaneh KhayamabedMohammad Hossein Nasr-Esfahani
Jul 27, 2020·Translational Animal Science·Madison L ButlerMegan M Rolf
Sep 14, 2019·Molecular Reproduction and Development·S Siddalingappa ArchanaStephen A Krawetz
Nov 3, 2021·Biopreservation and Biobanking·Samara Sandy Jerônimo MoreiraAlexandre Rodrigues Silva

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