Recombinant Aeromonas hydrophila outer membrane protein 48 (Omp48) induces a protective immune response against Aeromonas hydrophila and Edwardsiella tarda

Research in Microbiology
Rekha KhushiramaniI Karunasagar

Abstract

The gene coding for an outer membrane protein Omp48 of Aeromonas hydrophila isolated from an infected fish was cloned and sequenced. Analysis of nucleotide sequence showed the omp48 gene to be an adhesin encoding a protein of 426 amino acids with high identity to the omp48 gene of Aeromonas veronii, another fish pathogen. The gene belonged to the maltoporin group of porins and had high similarity to LamB porins of A. hydrophila, Aeromonas salmonicida and Vibrio parahaemolyticus. The expressed purified recombinant protein had an estimated molecular weight of 48 kDa. Further, rabbit hyperimmune sera against the recombinant protein reacted with A. hydrophila, Aeromonas sobria and A. veronii whole cell proteins at the region of 48 kDa, in western blotting. The recombinant protein was immunogenic in the fish Labeo rohita Hamilton. Fish immunized with recombinant protein, when challenged with virulent A. hydrophila and another bacterial fish pathogen, Edwardsiella tarda, showed relative percent survivals of 69 and 60, respectively. Our results suggest that Omp48 of A. hydrophila could be used as a potential vaccine candidate for protection not only against A. hydrophila infection, but also against the fish pathogen E. tarda.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Feb 1, 1992·Molecular Microbiology·H Nikaido
Jan 1, 1989·Critical Reviews in Microbiology·M Altwegg, H K Geiss
Aug 12, 2000·Fish & Shellfish Immunology·M H Rahman, K Kawai
Jun 7, 2003·Journal of Clinical Microbiology·Sharon L AbbottJ Michael Janda
Jun 17, 2003·International Journal of Systematic and Evolutionary Microbiology·M A YáñezA J Martínez-Murcia
Mar 12, 2004·Journal of Applied Microbiology·R C Vàzquez-JuárezF Ascencio
Sep 9, 2006·Protein Expression and Purification·Rekha KhushiramaniIddya Karunasagar
Jul 1, 2009·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Takamitsu SakaiTakaji Iida

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Citations

Dec 15, 2015·Journal of Fish Diseases·S Menanteau-Ledouble, M El-Matbouli
Jan 12, 2013·Journal of Fish Diseases·R Beaz-Hidalgo, M J Figueras
Aug 15, 2014·Critical Reviews in Food Science and Nutrition·Vandana BaliJohn F Kennedy
Aug 28, 2020·Frontiers in Immunology·Biswajit MaitiØystein Evensen
Aug 31, 2019·BioMed Research International·Alexanda MzulaGabriel M Shirima
Nov 10, 2020·Frontiers in Microbiology·Nahid AkterKammara Rajagopal
Aug 18, 2020·Letters in Applied Microbiology·S Hossain, G-J Heo
Aug 25, 2012·Veterinary Immunology and Immunopathology·Biswajit MaitiIndrani Karunasagar
Aug 3, 2021·International Journal of Peptide Research and Therapeutics·Furqan AwanYongjie Liu

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