PMID: 9438477Jan 23, 1998Paper

Recombinant allergen fragments as candidate preparations for allergen immunotherapy

The Journal of Allergy and Clinical Immunology
T ZeilerT Virtanen

Abstract

Lately, renewed interest has arisen in the new forms of allergen immunotherapy because they may offer alternatives for drug treatment. The purpose of this study was to develop a well-characterized preparation of the main respiratory cow dander allergen, Bos d 2, with attenuated allergenic activity. The immunologic characteristics of Bos d 2 preparations were studied by indirect IgE ELISA, ELISA inhibition, Western blotting, histamine release, skin prick tests, and the proliferation tests of allergen-specific T-cell clones. The complete recombinant Bos d 2 was observed to bind effectively, IgE of cow-allergic patients in indirect ELISA. In other experiments, the IgE-binding capacity of recombinant Bos d 2 proved to be lower compared with native Bos d 2. When the two overlapping recombinant fragments of Bos d 2 (corresponding amino acids 1-131 and 81-172, respectively) covering the whole molecule were compared with the complete recombinant Bos d 2 with several methods, only a low level of residual reactivity was observed. For example, recombinant fragments could not bind antibody at all in ELISA inhibition tests retaining, however, some reactivity in skin prick tests. In contrast, the fragments were able to stimulate vigorously B...Continue Reading

References

Jan 1, 1992·International Archives of Allergy and Immunology·J YlönenT Virtanen
Jan 1, 1992·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J YlönenT Virtanen
Sep 1, 1995·The Journal of Allergy and Clinical Immunology·T VirtanenS Romognani
May 1, 1995·Allergy·O Scheiner, D Kraft
Dec 1, 1994·Current Opinion in Immunology·S Romagnani
Jun 1, 1995·The Journal of Allergy and Clinical Immunology·J B SluntM D Chapman
Mar 1, 1994·The European Respiratory Journal·C E MappL M Fabbri
May 1, 1994·The Journal of Allergy and Clinical Immunology·J YlönenR Mäntyjärvi
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T J BrinerJ L Greenstein
Jan 1, 1996·American Journal of Respiratory and Critical Care Medicine·A OshibaE W Gelfand
Jun 1, 1996·The Journal of Allergy and Clinical Immunology·R MäntyjärviT Virtanen

❮ Previous
Next ❯

Citations

Feb 14, 2004·Methods : a Companion to Methods in Enzymology·M van Hage-Hamsten, G Pauli
Feb 14, 2004·Methods : a Companion to Methods in Enzymology·Susanne VrtalaRudolf Valenta
Nov 4, 2000·Biochimica Et Biophysica Acta·R MäntyjärviT Virtanen
Nov 5, 2002·Current Opinion in Immunology·Rudolf Valenta, Dietrich Kraft
Jun 20, 2000·Allergy·C A Akdis, K Blaser
Jun 26, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J KauppinenT Virtanen
Jun 26, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R ValentaH Grönlund
Jun 26, 2001·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P RuoppiA Taivainen
Mar 3, 2010·Annual Review of Immunology·Rudolf ValentaSusanne Vrtala
Jul 3, 2013·International Archives of Allergy and Immunology·Heidi R MyrsetMaaike M B W Dooper
Nov 4, 2000·Expert Opinion on Investigational Drugs·J M RollandR E O'Hehir
Apr 29, 2014·Journal of Proteomics·Ingrid MillerElisabetta Gianazza
Jun 12, 2010·Allergologia et immunopathologia·J M Lucas
Dec 30, 2008·Pharmacology & Therapeutics·Jennifer M RollandRobyn E O'Hehir
Oct 14, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S SaarelainenT Virtanen
Nov 17, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·W SmithW R Thomas
Jan 10, 2013·Scandinavian Journal of Immunology·S ParviainenT Virtanen
Oct 12, 2004·Immunology and Allergy Clinics of North America·Verena Niederberger, Rudolf Valenta
Oct 26, 2012·Human Vaccines & Immunotherapeutics·Marek JutelSylwia Smolinska
May 4, 2017·International Archives of Allergy and Immunology·Angelika Tscheppe, Heimo Breiteneder
Apr 6, 2002·International Immunology·Soili SaarelainenTuomas Virtanen
Apr 12, 2001·Allergy·T Virtanen
Mar 1, 2016·Allergy, Asthma & Immunology Research·Marek JutelSylwia Smolinska
Mar 10, 2015·Allergy, Asthma & Immunology Research·Marja Rytkönen-NissinenTuomas Virtanen
Feb 3, 2006·Expert Review of Vaccines·Verena Niederberger, Rudolf Valenta
Jul 28, 2020·International Journal of Molecular Sciences·Dulashi Withanage Dona, Cenk Suphioglu
Jan 16, 1999·The Journal of Biological Chemistry·J RouvinenR Mäntyjärvi
Jun 23, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·R Bredehorst, K David

❮ Previous
Next ❯

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.