PMID: 9176569May 1, 1997Paper

Recombinant and tissue-derived mouse BM-40 bind to several collagen types and have increased affinities after proteolytic activation

Cellular and Molecular Life Sciences : CMLS
P MaurerR Nischt

Abstract

The calcium-binding extracellular matrix protein BM-40 was obtained as a mouse cDNA product from a stably transfected kidney cell clone. Electrophoresis and N-terminal sequence analysis demonstrated absence of the proteolytic processing previously observed for a mouse tumour-derived BM-40. Yet the two forms of BM-40 were very similar in their CD spectra, their calcium-dependent change in alpha helix content and their immunological epitopes. In surface plasmon resonance assays, recombinant mouse BM-40 showed distinct binding to the triple-helical domains of collagens I, II, III, IV and V with Kd = 1-4 microM but no binding to collagen VI. These interactions were abolished in the presence of EDTA. Tissue-derived mouse BM-40, however, bound collagens I and IV with Kd = 0.1-0.2 microM. Activation of collagen binding to give a similar Kd could be achieved for recombinant mouse BM-40 by treatment with the matrix metalloproteinase collagenase-3. The major cleavage site was located in helix C of the extracellular calcium-binding module of BM-40 and other less prominent cleavages occurred close to the N-terminus. The sensitive helix C site was just one residue away from that sensitive to endogenous tissue proteolysis, suggesting that cl...Continue Reading

Citations

May 20, 2008·The Journal of Biological Chemistry·Camilla GiudiciRuggero Tenni
Jan 23, 2016·Journal of Molecular and Cellular Cardiology·Marieke Rienks, Anna-Pia Papageorgiou
Nov 19, 2015·Journal of Molecular and Cellular Cardiology·Margaux A Horn, Andrew W Trafford
May 6, 2006·European Journal of Oral Sciences·Yong-Hee P ChunJames P Simmer
Nov 3, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Hua WangShaoyi Jiang
Jul 2, 2015·European Journal of Heart Failure·Stephane HeymansJavier Díez
Oct 18, 2000·Molecular Cell Biology Research Communications : MCBRC·A D BradshawE H Sage
Sep 25, 2004·Journal of Cellular Physiology·Amber E HudsonJames A Bassuk
Jan 5, 2013·Circulation Research·Francis G SpinaleMichael R Zile
Mar 1, 2014·Circulation Research·Marieke RienksStephane Heymans
Jan 1, 2004·The Journal of Biological Chemistry·Harald O HambrockUrsula Hartmann
Mar 7, 2000·Journal of the American Society of Nephrology : JASN·Sebastian WalpenJosef Pfeilschifter

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.