Abstract
The tuberculosis (TB) pandemic remains a leading cause of human morbidity and mortality, despite widespread use of the only licensed anti-TB vaccine, bacille Calmette-Guerin (BCG). The protective efficacy of BCG in preventing pulmonary TB is highly variable; therefore, an effective new vaccine is urgently required. In the present study, we assessed the ability of novel recombinant BCG vaccine (rBCG) against Mycobacterium tuberculosis by using modern immunological methods. Enzyme-linked immunospot assays demonstrated that the rBCG vaccine, which coexpresses two mycobacterial antigens (Ag85B and CFP10) and human interleukin (IL)-12 (rBCG2) elicits greater interferon-γ (IFN-γ) release in the mouse lung and spleen, compared to the parental BCG. In addition, rBCG2 triggers a Th1-polarized response. Our results also showed that rBCG2 vaccination significantly limits M. tuberculosis H37Rv multiplication in macrophages. The rBCG2 vaccine surprisingly induces significantly higher tumor necrosis factor-α (TNF-α) production by peripheral blood mononuclear cells that were exposed to a nonmycobacterial stimulus, compared to the parental BCG. In this study, we demonstrated that the novel rBCG2 vaccine may be a promising candidate vaccine aga...Continue Reading
References
Nov 1, 1995·American Journal of Respiratory and Critical Care Medicine·F A al-KassimiE A Bamgboye
Jan 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·T F Brewer, G A Colditz
Mar 2, 1994·JAMA : the Journal of the American Medical Association·G A ColditzF Mosteller
Nov 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·M A HorwitzS Maslesa-Galic'
Jan 5, 2002·Trends in Microbiology·Pablo J BifaniBarry N Kreiswirth
Jun 18, 2002·Immunology and Cell Biology·Sarah YoungGlenn Buchan
Sep 20, 2005·Genome Research·Igor MokrousovOlga Narvskaya
Sep 12, 2006·Microbes and Infection·Ajay GroverG K Khuller
Feb 12, 2009·Molecular Ecology·Takashi Kanbe, Shin-ichi Akimoto
Mar 20, 2010·FEMS Immunology and Medical Microbiology·Lei ChenGuo-Zhi Wang
Sep 21, 2010·Cytometry. Part B, Clinical Cytometry·Erica SalernoElizabeth Raveche
Nov 19, 2010·Journal of Clinical Microbiology·Suporn PholwatEric Houpt
Feb 9, 2011·Antimicrobial Agents and Chemotherapy·Patricia J CampbellJames E Posey
Mar 30, 2011·Antimicrobial Agents and Chemotherapy·Claudia PlinkeStefan Niemann
Aug 5, 2011·Immunobiology·Antima GuptaSanjib Bhakta
Nov 1, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Shiang-Fen HuangMing-Chih Yu
Dec 14, 2011·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Chih-Wei LinHorng-Yunn Dou
Jan 12, 2012·PloS One·Neus CaceresPere-Joan Cardona
May 9, 2012·PloS One·Fan F KaoWarwick J Britton
Jul 19, 2012·PloS One·Yih-Yuan ChenHorng-Yunn Dou
Sep 1, 2012·PloS One·Jia-Ru ChangHorng-Yunn Dou
Sep 28, 2012·Clinical and Vaccine Immunology : CVI·Desta KassaDebbie van Baarle
Nov 7, 2012·The Journal of Antimicrobial Chemotherapy·R J ShortenT D McHugh
Dec 5, 2012·Antimicrobial Agents and Chemotherapy·M de VosT C Victor
Apr 26, 2013·Tuberculosis·Purva Bhatter, Nerges Mistry
Nov 14, 2013·Ecology and Evolution·Catalina Monzón-ArgüelloSofia Consuegra
Dec 5, 2013·Vaccine·Swati SinghSadhna Sharma
Citations
Jan 27, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·V K SinghB S Srivastava
Jul 14, 2020·Frontiers in Immunology·Shiu-Ju YangHorng-Yunn Dou
Dec 10, 2020·Vaccines·Abu Salim Mustafa
Jan 13, 2021·Vaccines·Abu Salim Mustafa
May 1, 2021·International Journal of Environmental Research and Public Health·Wei-Ju SuChin-Hui Yang