Abstract
Since BCG, the only vaccine widely used against tuberculosis (TB) in the world, provides varied protective efficacy and may not be effective for inducing long-term cellular immunity, it is in an urgent need to develop more effective vaccines and more potent immune strategies against TB. Prime-boost is proven to be a good strategy by inducing long-term protection. In this study, we tested the protective effect against Mycobacterium tuberculosis (Mtb) challenge of prime-boost strategy by recombinant BCG (rBCG) expressing PPE protein Rv3425 fused with Ag85B and Rv3425. Results showed that the prime-boost strategy could significantly increase the protective efficiency against Mtb infection, characterized by reduction of bacterial load in lung and spleen, attenuation of tuberculosis lesions in lung tissues. Importantly, we found that Rv3425 boost, superior to Ag85B boost, provided better protection against Mtb infection. Further research proved that rBCG prime-Rv3425 boost could obviously increase the expansion of lymphocytes, significantly induce IL-2 production by lymphocytes upon PPD stimulation, and inhibit IL-6 production at an early stage. It implied that rBCG prime-Rv3425 boost opted to induce Th1 immune response and provided...Continue Reading
References
Mar 2, 1994·JAMA : the Journal of the American Medical Association·G A ColditzF Mosteller
Oct 16, 2002·Annals of the Rheumatic Diseases·S H E Kaufmann
Nov 8, 2002·Journal of Chemotherapy·R SariS Ozen
Apr 25, 2003·Journal of Biosciences·Swathi Adindla, Lalitha Guruprasad
May 14, 2005·Microbes and Infection·Helen McShane, Adrian Hill
Apr 23, 2008·Microbiology and Immunology·Jiuling WangHonghai Wang
Aug 4, 2009·Current Opinion in Immunology·Andrea M Cooper
Aug 29, 2009·Vaccine·Yu LuoBingdong Zhu
Feb 9, 2011·Immunology and Cell Biology·Alessandra Mortellaro, Paola Ricciardi-Castagnoli
Feb 15, 2011·Clinical & Developmental Immunology·Samantha L Sampson
Mar 17, 2012·Bulletin of the World Health Organization·Matteo ZignolMario Raviglione
Aug 22, 2012·Human Vaccines & Immunotherapeutics·Jiuling WangHonghai Wang
Jun 26, 2013·Human Vaccines & Immunotherapeutics·David A Hokey, Ann Ginsberg
Jan 24, 2014·SpringerPlus·Prati Pal Singh, Amit Goyal
Jan 24, 2014·International Immunopharmacology·Rong JinJian-hua Zhang
Apr 23, 2014·Immunology and Cell Biology·Martin PalMartin Whitham
Oct 8, 2014·Molecular Immunology·I H ChowdhuryB Bahar
Jun 27, 2015·Acta Biochimica Et Biophysica Sinica·Enzhuo YangZheng W Chen
Citations
May 10, 2016·Tuberculosis·Aliabbas A HusainRajpal S Kashyap
Nov 28, 2018·Journal of Cellular Physiology·Wu LiJianping Xie
Mar 31, 2018·Experimental and Therapeutic Medicine·Huichang HuangHongbo Shen
Sep 12, 2019·Frontiers in Immunology·Iris ValdésLázaro Gil
Nov 20, 2016·Pathogens and Disease·Aarti RanaYusuf Akhter
Mar 17, 2017·Human Vaccines & Immunotherapeutics·Fu-Ying DaiLang Bao
Feb 21, 2018·Frontiers in Immunology·Natalie E Nieuwenhuizen, Stefan H E Kaufmann
Oct 4, 2019·DNA and Cell Biology·Xiaowen YuJianping Xie
Sep 8, 2021·Frontiers in Cellular and Infection Microbiology·Fanli YiYi Xie