Recombinant canine distemper virus strain Snyder Hill expressing green or red fluorescent proteins causes meningoencephalitis in the ferret.

Journal of Virology
Martin LudlowW Paul Duprex

Abstract

The propensity of canine distemper virus (CDV) to spread to the central nervous system is one of the primary features of distemper. Therefore, we developed a reverse genetics system based on the neurovirulent Snyder Hill (SH) strain of CDV (CDV(SH)) and show that this virus rapidly circumvents the blood-brain and blood-cerebrospinal fluid (CSF) barriers to spread into the subarachnoid space to induce dramatic viral meningoencephalitis. The use of recombinant CDV(SH) (rCDV(SH)) expressing enhanced green fluorescent protein (EGFP) or red fluorescent protein (dTomato) facilitated the sensitive pathological assessment of routes of virus spread in vivo. Infection of ferrets with these viruses led to the full spectrum of clinical signs typically associated with distemper in dogs during a rapid, fatal disease course of approximately 2 weeks. Comparison with the ferret-adapted CDV(5804P) and the prototypic wild-type CDV(R252) showed that hematogenous infection of the choroid plexus is not a significant route of virus spread into the CSF. Instead, viral spread into the subarachnoid space in rCDV(SH)-infected animals was triggered by infection of vascular endothelial cells and the hematogenous spread of virus-infected leukocytes from men...Continue Reading

References

Jul 1, 1988·Journal of Wildlife Diseases·E S WilliamsD W Belitsky
Jan 1, 1987·Acta Neuropathologica·M K Axthelm, S Krakowka
Jul 1, 1985·Journal of Comparative Pathology·B A Summers, M J Appel
Oct 1, 1974·The Journal of Infectious Diseases·B McCulloughJ Shadduck
Dec 1, 1974·Journal of Clinical Pathology·D R Purdham, P F Batty
Aug 16, 1969·The Veterinary Record·I F Keymer, H B Epps
Jan 1, 1984·Journal of Comparative Pathology·B A SummersM J Appel
Jul 1, 1993·Journal of Wildlife Diseases·P J DuignanJ R Geraci
Jun 1, 1997·Brain Research. Brain Research Reviews·G Allt, J G Lawrenson
Aug 10, 1956·Annals of the New York Academy of Sciences·J A BAKERG POPPENSIEK
Nov 12, 2003·Journal of Virology·Veronika von MesslingRoberto Cattaneo
Sep 16, 2006·Journal of Virology·Penny A RuddVeronika von Messling
Feb 27, 2007·Epilepsia·Nicola MarchiDamir Janigro
Aug 19, 2007·Journal of Virology·François BonamiVeronika von Messling
Sep 24, 2008·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Eleonora TavazziEnrico Marchioni
Nov 21, 2008·Veterinary Immunology and Immunopathology·A BeinekeW Baumgärtner
Nov 22, 2008·Journal of Biomedical Optics·Nikolaos C DeliolanisVasilis Ntziachristos
Dec 31, 2008·Journal of Neurovirology·Rennos FragkoudisJohn K Fazakerley
Aug 7, 2009·The Journal of General Virology·Phil ChambersW Paul Duprex
Dec 18, 2009·The Journal of General Virology·Penny A RuddVeronika von Messling
Oct 23, 2010·Journal of Wildlife Diseases·Kathy S QuigleyDale G Miquelle
Sep 22, 2011·Diseases of Aquatic Organisms·Giovanni Di GuardoClaudia Eleni

❮ Previous
Next ❯

Citations

Oct 8, 2015·Veterinary Microbiology·S ParidaA C Banyard
Feb 15, 2014·Current Opinion in Virology·Martin LudlowW Paul Duprex
Jun 20, 2013·Journal of Virological Methods·D Tien NguyenW Paul Duprex
Apr 23, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Renata da Fontoura BudaszewskiCláudio Wageck Canal
Oct 12, 2016·Viruses·Renata da Fontoura Budaszewski, Veronika von Messling
Dec 3, 2016·The Journal of General Virology·Angelika K LootsEstelle H Venter
Mar 16, 2019·The Journal of General Virology·Chen ChenLing Zhao
Mar 9, 2019·Virology Journal·Santiago Rendon-MarinJulian Ruiz-Saenz
May 20, 2021·Royal Society Open Science·Anet J N AneloneSarah K Spurgeon

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.