Recombinant carp IL-4/13B stimulates in vitro proliferation of carp IgM(+) B cells

Fish & Shellfish Immunology
Takuya YamaguchiTadaaki Moritomo

Abstract

Teleost IL-4/13B is a cytokine related to mammalian IL-4 and IL-13, of which hitherto the function had not been studied at the protein level. We identified an IL-4/13B gene in common carp (Cyprinus carpio) and expressed the recombinant protein (rcIL-4/13B). RcIL-4/13B was shown to stimulate proliferation of IgM(+) B cells, because after four days of stimulation the IgM(+) fraction of carp kidney and spleen leukocytes had formed many cell colonies, whereas such colonies were not found in the absence of rcIL-4/13B stimulation. After nine days of incubation with rcIL-4/13B these cells had proliferated to more than 3-to-7-fold higher numbers when compared to untreated cells. The proliferating cells contained a majority of IgM(+) cells but also other cells, as indicated by FACS and RT-PCR analyses. The important conclusion is that in fish not only IL-4/13A has B cell stimulating properties, as a previous publication has shown, but also IL-4/13B.

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Citations

May 28, 2016·Biology·Jun Zou, Christopher J Secombes
Mar 9, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tomoyuki OdakaToshiaki Miyadai
Aug 12, 2020·Scientific Reports·Annelieke S WentzelGeert F Wiegertjes
Dec 28, 2016·Fish & Shellfish Immunology·Ottavia BenedicentiChristopher J Secombes

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