PMID: 7538075Apr 1, 1995Paper

Recombinant chicken interferon from Escherichia coli and transfected COS cells is biologically active

European Journal of Biochemistry
U SchultzPeter Staeheli

Abstract

We have expressed a cDNA for virus-induced chick interferon in Escherichia coli. The product, a 19-kDa protein lacking the signal peptide, was purified to homogeneity from the bacterial inclusion bodies. Proteins in the insoluble fraction of bacterial lysates were dissolved in guanidine hydrochloride and subjected to chromatography on Q-Sepharose and MonoS columns. Purified recombinant chick interferon has a specific antiviral activity of approximately 10(8) IU/mg and is a powerful inducer of the interferon-responsive promoter of the chicken Mx gene. Culture medium of transfected COS cells expressing full-length chick interferon cDNA contained up to 5 x 10(4) IU antiviral activity/ml that could be neutralized by antibodies to purified recombinant chick interferon. The antibodies precipitated proteins of 23-28 kDa from the supernatants of transfected COS cells. Treatment with endoglycosidase F reduced the size of the immunoprecipitated proteins to approximately 20 kDa, demonstrating that chick interferon is a glycoprotein.

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Citations

Oct 1, 1996·Veterinary Immunology and Immunopathology·S K AgarwalJ Burnside
Apr 6, 2004·Developmental and Comparative Immunology·Ursula SchultzPeter Staeheli
Feb 26, 2000·Developmental and Comparative Immunology·C Siatskas, R Boyd
Mar 16, 2000·Developmental and Comparative Immunology·D L Suarez, S Schultz-Cherry
Jan 22, 1998·Veterinary Immunology and Immunopathology·K D SongJ Y Han
Nov 30, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·M J SekellickP I Marcus
Jan 19, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·P StaeheliB Kaspers
Aug 17, 2001·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·J W LowenthalP I Marcus
Mar 13, 2001·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·M H KogutK C Weining
Apr 1, 1997·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·R MarienfeldC Jungwirth
Jan 11, 2008·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Philip I Marcus
Jun 3, 2011·Journal of Virology·Benjamin SchusserSonja Härtle
May 27, 2008·Journal of Zhejiang University. Science. B·Li SongYi Li
Feb 9, 2008·Emerging Infectious Diseases·Philip I MarcusMargaret J Sekellick
Sep 5, 2001·Molecular and Cellular Biology·R HrdlickováH R Bose
Oct 31, 2015·Virology·Irene Lostalé-SeijoJavier Benavente
Mar 8, 2000·Cytokine·C SickK C Weining
Jul 22, 2016·The Journal of General Virology·Antje ReuterDennis Rubbenstroth
Oct 28, 2016·GM Crops & Food
Nov 29, 2016·The Journal of Veterinary Medical Science·Jun ZhaoJason Chen
Apr 1, 2010·Viruses·Antje ReuterPeter Staeheli
Dec 29, 2013·Journal of Virology·Antje ReuterDennis Rubbenstroth
Jul 22, 2016·Journal of Virology·Irene Lostalé-SeijoJavier Benavente
Oct 1, 1996·European Journal of Immunology·K C WeiningP Staeheli
Feb 9, 2005·The Journal of Veterinary Medical Science·Ruttapong RuttanapummaKazuaki Takehara
Apr 26, 1996·The Journal of Biological Chemistry·M SchwemmleU Schultz
Mar 29, 1996·The Journal of Biological Chemistry·C SickP Staeheli
Sep 27, 2020·Veterinary Immunology and Immunopathology·Youngsub LeeHyun S Lillehoj
Aug 17, 2021·Biotechnology and Bioprocess Engineering : BBE·Siddhesh B GhagVishwas A Bapat

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