PMID: 2101488Sep 1, 1990Paper

Recombinant cholera toxin B subunit and gene fusion proteins for oral vaccination

Research in Microbiology
J SanchezJ Holmgren

Abstract

The B subunit portion of cholera toxin (CTB) is a safe and effective oral immunizing agent in humans, affording protection against both cholera and diarrhoea caused by enterotoxigenic Escherichia coli producing heat-labile toxin (LT) (Clemens et al., 1986; 1988). CTB may also be used as a carrier of various "foreign" antigens suitable for oral administration. To facilitate large-scale production of CTB for vaccine development purposes, we have constructed recombinant overexpression systems for CTB proteins in which the CTB gene is under the control of strong foreign (non-cholera) promoters and in which it is also possible to fuse oligonucleotides to the CTB gene and thereby achieve overexpression of hybrid proteins (Sanchez and Holmgren, 1989; Sanchez et al., 1988). We here expand these findings by describing overexpression of CTB by a constitutive tacP promoter as well as by the T7 RNA-polymerase promoter, and also by describing gene fusions leading to overexpression of several hybrid proteins between heat-stable E. coli enterotoxin (STa)-related peptides to either the amino or carboxy ends of CTB. Each of the hybrid proteins, when tested as immunogens in rabbits, stimulated significant anti-STa as well as anti-CTB antibody fo...Continue Reading

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Citations

Oct 1, 1994·Protein Science : a Publication of the Protein Society·C L VerlindeW G Hol
Feb 15, 2011·Plant Cell Reports·Sergio Rosales-MendozaÁngel G Alpuche-Solís
Nov 17, 2007·Biotechnology Letters·Anisia J SilvaJorge A Benitez
Dec 1, 1991·FEMS Microbiology Immunology·J Holmgren
Mar 31, 1998·Journal of Immunological Methods·S LiljeqvistM Murby
Nov 24, 1999·Immunological Reviews·C CzerkinskyJ B Sun
Mar 17, 2010·Infection and Immunity·Arne TaxtPål Puntervoll
Jan 1, 1996·Journal of Drug Targeting·A J Almeida, H O Alpar
Oct 16, 2004·Expert Review of Vaccines·Donata R SizemoreUna S Ryan
Aug 24, 2013·Toxins·Antonella Antignani, David Fitzgerald
Jan 1, 1995·Clinical Microbiology Reviews·J B KaperM M Levine
Jan 31, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Shiv K SharmaRoger M Leblanc

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