Recombinant CP40 from Corynebacterium pseudotuberculosis confers protection in mice after challenge with a virulent strain

Vaccine
Daniela Droppa-AlmeidaFrancine F Padilha

Abstract

Caseous Lymphadenitis (CLA) is a contagious, infectious, chronic disease caused by Corynebacterium pseudotuberculosis, which affects mainly sheep and goats. The clinical prevalence of CLA in Brazil is 30%, resulting in decreased milk production, weight loss, and unusable meat and leather. Prophylaxis is based on vaccination; however, current vaccinations do not offer effective protection against the infection, which makes the development of a new vaccine essential to control this disease. Here, we developed a recombinant vaccine based on CP40 protein (rCP40) combined with an adjuvant (Freund's complete adjuvant or saponin) and evaluated its efficacy in a murine model of CLA. Female BALB/c mice were used in an immunization assay. rCP40 induced high levels of IgG2a and IgG2b antibodies. After challenge with a virulent strain of C. pseudotuberculosis C57 (10(4)CFU/mL), the levels of IgG2a and IgG2b were sustained, indicating a Th1 response. The groups immunized with rCP40 protein (GES and GEF groups) showed 100% protection and was statistically significant in the GES and GEF groups (p<0.037 and p<0.0952, respectively). The results indicated the recombinant protein CP40 induced an specific immune response in mice that was able to a...Continue Reading

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Citations

Nov 9, 2016·BMC Microbiology·Azadeh ShadnezhadMattias Collin
Jun 26, 2018·The Journal of Veterinary Medical Science·Eliane Macedo Sobrinho SantosKattyanne Souza Costa
Jul 10, 2019·Microbial Biotechnology·Thiago Doria BarralRicardo Dias Portela
Oct 28, 2017·Frontiers in Microbiology·Alberto OliveiraAlice R Wattam

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