Abstract
Caseous Lymphadenitis (CLA) is a contagious, infectious, chronic disease caused by Corynebacterium pseudotuberculosis, which affects mainly sheep and goats. The clinical prevalence of CLA in Brazil is 30%, resulting in decreased milk production, weight loss, and unusable meat and leather. Prophylaxis is based on vaccination; however, current vaccinations do not offer effective protection against the infection, which makes the development of a new vaccine essential to control this disease. Here, we developed a recombinant vaccine based on CP40 protein (rCP40) combined with an adjuvant (Freund's complete adjuvant or saponin) and evaluated its efficacy in a murine model of CLA. Female BALB/c mice were used in an immunization assay. rCP40 induced high levels of IgG2a and IgG2b antibodies. After challenge with a virulent strain of C. pseudotuberculosis C57 (10(4)CFU/mL), the levels of IgG2a and IgG2b were sustained, indicating a Th1 response. The groups immunized with rCP40 protein (GES and GEF groups) showed 100% protection and was statistically significant in the GES and GEF groups (p<0.037 and p<0.0952, respectively). The results indicated the recombinant protein CP40 induced an specific immune response in mice that was able to a...Continue Reading
References
Dec 1, 1965·New Zealand Veterinary Journal·R D Jolly
Jan 1, 1994·Vaccine·G B LipfordK Heeg
May 1, 1997·Veterinary Immunology and Immunopathology·J R BogdanJ A Ellis
Nov 5, 2003·American Journal of Human Biology : the Official Journal of the Human Biology Council·Sidia M Callegari-JacquesMara H Hutz
May 7, 2004·Veterinary Immunology and Immunopathology·Thiru VanniasinkamMichael W Heuzenroeder
Jul 27, 2004·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·C R R RamosP L Ho
Oct 14, 2004·Immunology and Cell Biology·Nikolai Petrovsky, Julio César Aguilar
Jun 30, 2006·Vaccine·Michael C FontaineWilliam Donachie
Mar 6, 2007·Vaccine·J C Aguilar, E G Rodríguez
Feb 16, 2008·Critical Reviews in Food Science and Nutrition·Jack N Losso
Feb 7, 2009·Expert Review of Vaccines·Fernanda A DorellaVasco Azevedo
Aug 12, 2009·Research in Veterinary Science·N SeyffertV Azevedo
Nov 10, 2009·Vaccine·Eva VeronesiPhilip S Mellor
Jan 5, 2011·BMC Genomics·Eva TrostAndreas Tauch
Jan 11, 2011·The Veterinary Clinics of North America. Food Animal Practice·Peter A Windsor
Feb 12, 2011·PloS One·Saumyabrata MazumderNahid Ali
Jul 5, 2011·Experimental Parasitology·Wiebke HartmannMinka Breloer
Jul 22, 2011·BMC Research Notes·Marcilia P CostaVasco Azevedo
Nov 10, 2011·BMC Veterinary Research·Alessandro S GuimarãesAurora M G Gouveia
Feb 22, 2012·Experimental Parasitology·Jaime R Adame-GallegosRichard J Pleass
Mar 20, 2012·Vaccine·Joanne M LumsdenAnjali Yadava
Mar 24, 2012·Immunobiology·Rajdeep ChoudhuryTapati Chakraborti
Oct 2, 2012·Experimental and Molecular Pathology·Farinaz Safavi, Abdolmohamad Rostami
Dec 4, 2012·Journal of Biotechnology·Siomar C SoaresAndreas Tauch
Citations
Nov 9, 2016·BMC Microbiology·Azadeh ShadnezhadMattias Collin
May 19, 2017·Journal of Medical Microbiology·Alexandre Antunes BrumSibele Borsuk
Jun 26, 2018·The Journal of Veterinary Medical Science·Eliane Macedo Sobrinho SantosKattyanne Souza Costa
Jul 10, 2019·Microbial Biotechnology·Thiago Doria BarralRicardo Dias Portela
Feb 2, 2018·PloS One·Ângela Sena-LopesSibele Borsuk
Aug 10, 2017·Frontiers in Cellular and Infection Microbiology·Wanderson M SilvaVasco Azevedo
Oct 28, 2017·Frontiers in Microbiology·Alberto OliveiraAlice R Wattam
Jan 25, 2021·Microbial Pathogenesis·Rodrigo Barros de PinhoSibele Borsuk
Nov 17, 2020·Vaccine·Mara Thais de Oliveira SilvaSibele Borsuk
Apr 6, 2021·Vaccine·Francisco Silvestre Brilhante BezerraSibele Borsuk
Aug 11, 2021·Veterinary Medicine International·Daniela Droppa-AlmeidaFrancine Ferreira Padilha
Oct 9, 2021·Applied Microbiology and Biotechnology·Mara Thais de Oliveira SilvaSibele Borsuk
Nov 30, 2021·PloS One·Daniela Droppa-AlmeidaFrancine Ferreira Padilha