Recombinant Culicoides obsoletus complex allergens stimulate antigen-specific T cells of insect bite hypersensitive Shetland ponies in vitro

Veterinary Dermatology
C MeulenbroeksEdwin Tijhaar

Abstract

Ponies may suffer from Insect bite hypersensitivity (IBH), an allergic IgE-mediated pruritic skin disorder, induced by allergens from biting midges of the Culicoides spp. To determine whether recombinant Culicoides obsoletus allergens are able to activate T cells of ponies exposed to C. obsoletus and whether these allergen-specific responses differ between IBH-affected and healthy ponies. Ten IBH-affected Shetland ponies and 10 age-matched healthy controls taken from the same stables, to ensure similar exposure to midges. Peripheral blood mononuclear cells (PBMC) were cultured with two different pools of recombinant C. obsoletus complex allergens to expand the allergen-specific T cells. These PBMC cultures were subsequently co-cultured with mature dendritic cells (DCs) loaded with the same antigens. Induction of Th1, Th2 and regulatory T (Treg) cells in these DC/PBMC co-cultures was assessed by analysis of IFN-γ, IL-4, IL-10 and FoxP3 expression levels using quantitative RT-PCR and phenotyping by flow cytometry. Recombinant C. obsoletus allergens increased IFN-γ mRNA expression levels, percentages of IFN-γ expressing (Th1) cells and CD25(high) FoxP3(+) IL-10(+) Tregs compared to unstimulated DC/PBMC co-cultures. Stimulation of ...Continue Reading

References

Jul 1, 1990·Equine Veterinary Journal·V A Fadok, E C Greiner
Sep 1, 1995·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S M McHughP W Ewan
Jan 5, 2002·Equine Veterinary Journal·A van der HaegenE Marti
Jul 28, 2005·Developmental and Comparative Immunology·Bettina Wagner
Aug 3, 2007·International Archives of Allergy and Immunology·E HamzaE Marti
Aug 21, 2007·Veterinary Immunology and Immunopathology·Sherif IbrahimFalko Steinbach
Feb 12, 2010·European Journal of Immunology·Oscar PalomaresCezmi A Akdis
Jun 28, 2011·Veterinary Immunology and Immunopathology·Eman HamzaEliane Marti
Aug 22, 2012·Veterinary Immunology and Immunopathology·Nathalie M A van der MeideEdwin Tijhaar
Dec 12, 2012·Veterinary Immunology and Immunopathology·C MeulenbroeksT Willemse
Apr 9, 2013·Veterinary Immunology and Immunopathology·Nathalie M A van der MeideEdwin Tijhaar
Jul 31, 2013·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·E HamzaE Marti

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.