Recombinant cyclophilins lack nuclease activity

Journal of Bacteriology
Angel Manteca, Jesús Sánchez

Abstract

Several single-domain prokaryotic and eukaryotic cyclophilins have been identified as also being unspecific nucleases with a role in DNA degradation during the lytic processes that accompany bacterial cell death and eukaryotic apoptosis. Evidence is provided here that the supposed nuclease activity of human and bacterial recombinant cyclophilins is due to contamination of the proteins by the host Escherichia coli endonuclease and is not an intrinsic property of these proteins.

References

Jul 14, 1968·Journal of Molecular Biology·H Dürwald, H Hoffmann-Berling
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·T NagataA Muraguchi
Nov 4, 2000·Medicinal Research Reviews·M T Ivery
May 22, 2002·Nature Structural Biology·Cordelia Schiene-FischerGunter Fischer

❮ Previous
Next ❯

Citations

Aug 17, 2005·Nature Reviews. Molecular Cell Biology·Kumiko Samejima, William C Earnshaw
Sep 28, 2010·Antioxidants & Redox Signaling·Irina F Sevrioukova
Jul 6, 2010·Cellular and Molecular Life Sciences : CMLS·Andrzej Galat, Jacqueline Bua
Jun 29, 2011·Critical Reviews in Microbiology·Amruta Pramod Joshi, Sumedha Sharad Deshmukh

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis