Recombinant cytochromes c biogenesis systems I and II and analysis of haem delivery pathways in Escherichia coli

Molecular Microbiology
Robert FeissnerR G Kranz

Abstract

Genetic analysis has indicated that the system II pathway for c-type cytochrome biogenesis in Bordetella pertussis requires at least four biogenesis proteins (CcsB, CcsA, DsbD and CcsX). In this study, the eight genes (ccmA-H) associated with the system I pathway in Escherichia coli were deleted. Using B. pertussis cytochrome c4 as a reporter for cytochromes c assembly, it is demonstrated that a single fused ccsBA polypeptide can replace the function of the eight system I genes in E. coli. Thus, the CcsB and CcsA membrane complex of system II is likely to possess the haem delivery and periplasmic cytochrome c-haem ligation functions. Using recombinant system II and system I, both under control of IPTG, we have begun to study the capabilities and characteristics of each system in the same organism (E. coli). The ferrochelatase inhibitor N-methylprotoporphyrin was used to modulate haem levels in vivo and it is shown that system I can use endogenous haem at much lower levels than system II. Additionally, while system I encodes a covalently bound haem chaperone (holo-CcmE), no covalent intermediate has been found in system II. It is shown that this allows system I to use holo-CcmE as a haem reservoir, a capability system II does no...Continue Reading

References

Feb 1, 1992·Genes & Development·D L BeckmanR G Kranz
Sep 1, 1985·Journal of Bacteriology·M D Moody, H A Dailey
Nov 1, 1995·Canadian Journal of Microbiology·M F AlexeyevT P Croughan
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D L Beckman, R G Kranz
Mar 1, 1996·The Journal of Biological Chemistry·Z Xie, S Merchant
Mar 1, 1996·Journal of Bacteriology·G SunF M Hulett
Nov 1, 1996·Journal of Bacteriology·B S GoldmanR G Kranz
Dec 1, 1996·European Journal of Biochemistry·C CavazzaM Bruschi
Feb 12, 1998·The Journal of Biological Chemistry·K InoueO A Sodeinde
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·B S GoldmanR G Kranz
May 15, 1998·Trends in Biochemical Sciences·M D PageS J Ferguson
Aug 26, 1998·Science·H SchulzL Thöny-Meyer
Aug 28, 1998·Molecular Microbiology·R KranzS Merchant
Oct 14, 1998·FEMS Microbiology Letters·E ReidJ A Cole
Mar 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·J W Trauger, C T Walsh
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Jun 9, 2000·Molecular Microbiology·N E Le BrunL Hederstedt
Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Oliver DaltropStuart J Ferguson
Mar 15, 2003·The Journal of Biological Chemistry·Lýğur S ErlendssonNick E Le Brun
Apr 4, 2003·Analytical Biochemistry·Robert FeissnerRobert G Kranz
Dec 22, 2004·Accounts of Chemical Research·Julie M StevensStuart J Ferguson
Jun 7, 2005·Journal of Bacteriology·Robert E FeissnerRobert G Kranz

❮ Previous
Next ❯

Citations

Nov 10, 2011·Biochemistry·Brian San FranciscoRobert G Kranz
Nov 26, 2008·Natural Product Reports·Sarah E J Bowman, Kara L Bren
Jun 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Elaine R Frawley, Robert G Kranz
Jun 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sabeeha S Merchant
Jul 16, 2010·The Journal of Biological Chemistry·Stéphane T GabillyPatrice P Hamel
May 15, 2010·The Journal of Biological Chemistry·Alan D GoddardStuart J Ferguson
May 2, 2013·Acta Crystallographica. Section D, Biological Crystallography·Ji Young YoonSe Won Suh
Mar 11, 2008·Journal of Bacteriology·Cynthia L Richard-FogalRobert G Kranz
Sep 2, 2009·Microbiology and Molecular Biology Reviews : MMBR·Robert G KranzElaine R Frawley
Feb 22, 2014·Photosynthesis Research·Trevor S KasheyKevin E Redding
Nov 24, 2007·Mitochondrion·P GiegéG Bonnard
Jul 25, 2009·The EMBO Journal·Cynthia L Richard-FogalRobert G Kranz
Apr 9, 2008·The FEBS Journal·James W A AllenMichael L Ginger
Oct 1, 2011·The FEBS Journal·Jörg Simon, Lars Hederstedt
Feb 12, 2014·Journal of Molecular Biology·Brian San Francisco, Robert G Kranz
Jul 6, 2010·Journal of Molecular Biology·Cynthia Richard-Fogal, Robert G Kranz
Sep 29, 2011·Biochimica Et Biophysica Acta·Cynthia L Richard-FogalRobert G Kranz
Jun 16, 2015·Trends in Biochemical Sciences·Shalon E BabbittRobert G Kranz
Jun 25, 2015·Frontiers in Microbiology·Katarzyna M Bocian-OstrzyckaElżbieta K Jagusztyn-Krynicka
Mar 19, 2014·Biochimica Et Biophysica Acta·Andreia F Verissimo, Fevzi Daldal
Apr 22, 2008·Biochimica Et Biophysica Acta·Stuart J FergusonIan B Robertson
Jul 29, 2008·Biochimica Et Biophysica Acta·Patrice HamelGéraldine Bonnard
Aug 21, 2007·Biochimica Et Biophysica Acta·Jong-Hoon LeeMilton H Saier
Nov 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Brian San FranciscoRobert G Kranz
Aug 5, 2016·Biochemistry·Paul A SigalaDaniel E Goldberg
Jul 11, 2006·Molecular Microbiology·Robert E FeissnerRobert G Kranz
Jun 28, 2007·The Journal of Biological Chemistry·Stephan BöhmKai-Hong Zhao
Jun 28, 2012·Molecular BioSystems·Rui HeIkuro Abe
Jun 7, 2007·The Journal of Biological Chemistry·Naganand RayapuramGéraldine Bonnard
Jan 26, 2011·Metallomics : Integrated Biometal Science·Julie M Stevens
Feb 16, 2017·Proceedings of the National Academy of Sciences of the United States of America·Deanna L MendezRobert G Kranz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.