PMID: 8601726Feb 1, 1996Paper

Recombinant desmoglein 3 has the necessary epitopes to adsorb and induce blister-causing antibodies

The Journal of Investigative Dermatology
O MemarB S Prabhakar

Abstract

The development of an animal model for studying the pathogenesis of pemphigus vulgaris (PV) has been hampered by the unavailability of the purified full-length autoantigen desmoglein 3 (Dsg 3).Therefore, we expressed Dsg 3 using a baculovirus expressed system. The expressed protein was identified as Dgs 3 by its reactivity with a pan-cadherin anti-serum, an anti-serum to a Dsg 3 synthetic peptide, or patient serum, and by amino-terminal sequencing. Carbohydrate analysis showed that recombinant Dsg 3 was glycosylated. While a majority of the recombinant protein was cell associated, by immunoprecipitation, some Dsg 3 was demonstrated in the medium. The Dgs 3 could adsorb out blister-causing antibodies from patient sera. Rabbit anti- Dsg 3 antibodies induced by the recombinant Dsg 3 showed specific binding to intercellular spaces of monkeys esophagus by indirect immunofluorescence. Moreover, these antibodies induced PV-like blisters in neonatal mice and weakly bound perilesional epidermis. Availability of large quantities of relatively pure Dsg 3 should now facilitate studies aimed at understanding Dsg 3 structure and pathogenesis of PV, with implications for developing specific immunotherapies.

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Citations

Feb 18, 1999·The Journal of Clinical Investigation·M G MahoneyJ R Stanley
May 29, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·E Dabelsteen
Nov 15, 2006·Autoimmunity·Robert Gniadecki
Nov 14, 2001·Clinics in Dermatology·P Martel, P Joly
May 8, 1999·The Journal of Investigative Dermatology·X DingZ Liu
Oct 16, 2019·The Ocular Surface·Omeed MemarAli R Djalilian

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