Recombinant DNA probes reveal simultaneous infection of tsetse flies with different trypanosome species

Molecular and Biochemical Parasitology
P A Majiwa, L H Otieno


The utility of recombinant DNA probes in the detection of natural trypanosome infection of tsetse flies has been assessed in Lambwe Valley, near the shores of Lake Victoria, Kenya. The tsetse flies were surveyed during two different seasons in 1988. Three different probes used each contained highly repetitive DNA sequences specific for a species or subspecies of trypanosomes of the Nanomonas subgenus. A fourth probe contained repetitive sequences common to trypanosome species of the Trypanozoon subgenus. Mixed mature or immature infections were detected in a variety of combinations in different individual tsetse flies. Such infections were detected in both the guts and mouthparts of some tsetse flies. Simultaneous natural infection of tsetse with the savannah type Trypanosoma congolense and Kilifi type T. congolense, T. congolense and Trypanosoma brucei or T. congolense and Trypanosoma simiae were demonstrated. The probes have thus been used to demonstrate the presence of Lambwe Valley, south-western Kenya, of a type of T. congolense first observed among trypanosome isolates obtained from sentinel cattle exposed to natural infection on a ranch at Kilifi on the Kenya coast. This type of T. congolense appears not to be confined t...Continue Reading


Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 1, 1986·Parasitology·P A MajiwaG Matthyssens
Jun 20, 1987·Journal of Molecular Biology·N B MurphyE Pays
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein

❮ Previous
Next ❯


Nov 1, 1992·International Journal for Parasitology·D K MasigaW C Gibson
Mar 1, 1996·Veterinary Parasitology·D K MasigaW C Gibson
Jun 12, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·Wendy Gibson
Dec 23, 1998·Transactions of the Royal Society of Tropical Medicine and Hygiene·P TrucG Cuny
Apr 13, 2000·Veterinary Parasitology·D O KihuraniS M Mbiuki
Nov 26, 2003·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Imna MaleleWendy Gibson
Nov 14, 2003·Kinetoplastid Biology and Disease·Wendy Gibson
Dec 19, 2012·Research in Veterinary Science·Michael I TakeetIkhide G Imumorin
Feb 17, 2015·The Onderstepoort Journal of Veterinary Research·Makhosazana Y MotloangAbdalla A Latif
Apr 14, 2017·BMC Veterinary Research·Reta D AbdiLuc Duchateau
Oct 1, 1996·Annals of Tropical Medicine and Parasitology·J P Gouteux, W C Gibson

❮ Previous
Next ❯

Related Concepts

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.