Recombinant DNA vaccines protect against tumors that are resistant to recombinant vaccinia vaccines containing the same gene

Gene Therapy
C H ChenT-C Wu

Abstract

Antigen-specific cancer immunotherapy involves the delivery of tumor-associated antigen to the host for the generation of tumor-specific immune responses and antitumor effects. We hypothesized that different delivery systems may influence the pattern of antigen-specific immune response and the outcome of antitumor effect. We therefore evaluated recombinant vaccinia virus and naked DNA for the generation of antigen-specific immune responses and antitumor effects. We previously found that recombinant vaccinia and naked DNA vaccines containing the chimeric Sig/E7/LAMP-1 gene were capable of controlling the growth of HPV-16 E7-expressing tumor cells (TC-1). In this study, we performed a head-to-head comparison of optimized delivery of Sig/E7/LAMP-1 vaccinia and DNA vaccines using dose-escalating tumor challenge. At a dose of 1 x 10(6) TC-1 cells per mouse, Sig/E7/LAMP-1 DNA provided 100% protection against subcutaneous growth of tumors, while Vac-Sig/E7/LAMP-1 protected only 40% of the mice. Furthermore, Sig/E7/LAMP-1 DNA vaccines are capable of protecting against challenge with a more stringent subclone of TC-1 (TC-1 P2) established from TC-1 tumors that survived initial Sig/E7/LAMP-1 vaccinia vaccination. Immunological assays rev...Continue Reading

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Citations

Nov 13, 2004·The Journal of Gene Medicine·Gérald J Prud'homme
Jul 21, 2005·Cancer Immunology, Immunotherapy : CII·Min Yu, Olivera J Finn
Jan 21, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yu-Qian ZhangChien-Fu Hung
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Jul 25, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Harjot K Saini, Naranjan S Dhalla
Mar 23, 2002·Experimental Biology and Medicine·Katharina HauptKlauss Mann

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