Recombinant egg drop syndrome subunit vaccine offers an alternative to virus propagation in duck eggs

Avian Pathology : Journal of the W.V.P.A
B GutterJ Pitcovski

Abstract

Egg drop syndrome (EDS) virus vaccines are routinely produced in embryonated duck eggs (Solyom et al., 1982). This procedure poses the risk of dissemination of pathogens, such as avian influenza virus, as the eggs used are not from specific pathogen free birds. To address this problem, the knob and part of the shaft domain of the fibre protein of the EDS virus (termed knob-s) were expressed in Escherichia coli and assessed as a subunit vaccine. A single vaccination with the recombinant protein induced the production of anti-EDS virus antibodies, as detected by haemagglutination inhibition, enzyme-linked immunosorbent assay and virus neutralization tests, for at least 20 weeks. A positive correlation was demonstrated between these three assays. A dose-response assessment showed that the vaccine was effective over the range of 2 to 64 microg protein per dose. Two vaccinations with the recombinant protein, administered before the onset of lay, induced high haemagglutination inhibition antibody titres, comparable with those induced by an inactivated whole-virus vaccine. The vaccine did not have any adverse effects on egg production, quality or weight. The present study has shown that two vaccinations with the recombinant knob-s pro...Continue Reading

References

Aug 1, 1965·Journal of Molecular Biology·R C Valentine, H G Pereira
Jun 1, 1994·Journal of Virology·N LouisJ Chroboczek
Jul 7, 2005·Vaccine·J PitcovskiB Gutter
Nov 28, 2006·Vaccine·Dennis J Alexander
Jan 1, 1980·Avian Pathology : Journal of the W.V.P.A·W BaxendaleI McPherson

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
ELISA
enzyme-linked immunosorbent assay

Software Mentioned

JMP

Related Concepts