Recombinant epoetins do not stimulate tumor growth in erythropoietin receptor-positive breast carcinoma models

Molecular Cancer Therapeutics
Kenneth R LaMontagneFrancis X Farrell

Abstract

We investigated the significance of erythropoietin receptor (EPOR) expression following treatment with recombinant human erythropoietin (rHuEPO; epoetin alpha) and the effect of recombinant epoetins (epoetin alpha, epoetin beta, and darbepoetin alpha) alone or in combination with anticancer therapy on tumor growth in two well-established preclinical models of breast carcinoma (MDA-MB-231 and MCF-7 cell lines). Expression and localization of EPOR under hypoxic and normoxic conditions in MDA-MB-231 and MCF-7 cells were evaluated by immunoblotting, flow cytometry, and immunohistochemistry. EPOR binding was evaluated using [125I]rHuEPO. Proliferation, migration, and signaling in MDA-MB-231 and MCF-7 cells following treatment with rHuEPO were evaluated. Tumor growth was assessed following administration of recombinant epoetins alone and in combination with paclitaxel (anticancer therapy) in orthotopically implanted MDA-MB-231 and MCF-7 breast carcinoma xenograft models in athymic mice. EPOR expression was detected in both tumor cell lines. EPOR localization was found to be exclusively cytosolic and no specific [125I]rHuEPO binding was observed. There was no stimulated migration, proliferation, or activation of mitogen-activated prot...Continue Reading

References

Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A AnagnostouM Steiner
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·A AnagnostouC T Noguchi
May 20, 1999·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·F P BarboneL K Jolliffe
Sep 25, 1999·Growth Factors·P A Tilbrook, S P Klinken
Aug 1, 2000·Kidney International·C Westenfelder, R L Baranowski
Nov 30, 2000·Melanoma Research·E SelzerB Jansen
Jun 2, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J L GabriloveL H Einhorn
Aug 18, 2001·Seminars in Oncology·J Carles, M Nogué
May 16, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Anders OsterborgUNKNOWN Epoetin Beta Hematology Study Group
Jul 16, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Murat O ArcasoyZishan A Haroon
Aug 22, 2002·Journal of the National Cancer Institute·Johan VansteenkisteUNKNOWN Aranesp 980297 Study Group
Sep 28, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J Douglas RizzoUNKNOWN American Society of Clinical Oncology. American Society of Hematology
Mar 11, 2003·The Oncologist·Terry Lappin
Jul 25, 2003·British Journal of Haematology·Michael HedenusUNKNOWN Darbepoetin Alfa 20000161 Study Group
Oct 18, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Sandeep BatraPunam Malik
Feb 11, 2004·Archives of Internal Medicine·David H HenryRobert Provenzano
Sep 29, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Thomas E WitzigRoscoe F Morton
Dec 14, 2004·The Oncologist·Francis Farrell, Adrian Lee
Dec 14, 2004·The Oncologist·Heinz Ludwig
Mar 4, 2005·The American Journal of Pathology·Suresh M KumarXiaowei Xu
Jun 21, 2005·Nature Reviews. Cancer·Jerry L Spivak
Aug 10, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Brian Leyland-JonesEls Vercammen

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Citations

Aug 1, 2008·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Jaime Sanz Ortiz
Jun 15, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Duong H NguyenAladar A Szalay
Apr 10, 2008·Cardiovascular Research·Ian B CoplandJacques Galipeau
Feb 4, 2010·Blood·Susan SwiftSteven Elliott
Jul 19, 2013·PloS One·Steve ElliottCarol Johnson
Aug 1, 2012·Biologics : Targets & Therapy·Steve Elliott, Angus M Sinclair
Jan 18, 2014·The Journal of Clinical Investigation·Bing ZhouWilliam Y Kim
Dec 18, 2013·Annals of Hematology·Steve ElliottWolfgang Jelkmann
Apr 4, 2015·Annual Review of Virology·Winnie M Chan, Grant McFadden
Jun 6, 2009·Journal of Cancer Research and Clinical Oncology·Cristiana Lo NigroMarco Merlano
Dec 19, 2009·Cancer radiothérapie : journal de la Société française de radiothérapie oncologique·E Le FurO Pradier
Jun 19, 2009·The Journal of Investigative Dermatology·Alireza MirmohammadsadeghUlrich R Hengge
Jul 16, 2008·Leukemia Research·Domenico Ribatti
Apr 18, 2008·Biochemical and Biophysical Research Communications·Agneta Månsson BrobergChrister Sylvén
Apr 25, 2008·Critical Reviews in Oncology/hematology·Wolfgang JelkmannArthur J Sytkowski
Jan 2, 2007·Critical Reviews in Oncology/hematology·Morgan Milano, Maurice Schneider
Apr 25, 2009·British Journal of Haematology·Francesca Merchionne, Franco Dammacco
Nov 9, 2007·International Journal of Cancer. Journal International Du Cancer·Magdalena LaugschWolfgang Jelkmann
Jan 27, 2007·European Journal of Haematology·Wolfgang Jelkmann
Apr 28, 2007·Stem Cells·C Anthony Blau
May 15, 2012·Leukemia Research·Howard S OsterMoshe Mittelman
Feb 8, 2014·Biochemical and Biophysical Research Communications·Susann ReinbotheSven Påhlman
Dec 4, 2010·Experimental Biology and Medicine·James W Fisher
Jul 29, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Brandon K Hadland, Gregory D Longmore
Sep 26, 2009·The Oncologist·Jerry L SpivakHeinz Ludwig
Sep 26, 2009·The Oncologist·Matti Aapro, Jerry L Spivak
Jul 4, 2008·The Oncologist·Ferdinando Mannello, Gaetana A M Tonti
Oct 12, 2007·The Journal of Pharmacology and Experimental Therapeutics·Kyoung-Han KimPeter H Backx
Jun 19, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Parviz KokhaeiAnders Osterborg
Jul 2, 2009·Molecular Cancer Research : MCR·Jee-Yeong JeongLaurie Feldman
Aug 5, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Murat O Arcasoy

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