PMID: 15228161Jul 2, 2004Paper

Recombinant erythropoietin and analogues: a challenge for doping control

Therapeutic Drug Monitoring
J A PascualJ Segura

Abstract

Erythropoietin (EPO) increases the number of circulating erythrocytes and thus muscle oxygenation. The availability of the recombinant protein (rEPO) has increased the risk of its illegal use in sports, its detection being a difficult challenge. Five different hematopoietic parameters were initially chosen as indirect markers of rEPO abuse: concentration of serum EPO, concentration of serum-soluble transferrin receptors (sTFr), hematocrit, percentage of reticulocytes, and percentage of macrocytes. New models considering only hemoglobin, serum EPO concentration, and percentage of reticulocytes are simpler and seem to be more sensitive when low doses of rEPO are used. A more direct method of urine analysis (isoelectrofocusing, double blotting, and chemiluminescent detection) based on the charge differences between rEPO and endogenous EPO, related to their carbohydrate composition, provides proof of rEPO use. Furthermore, this approach permits the detection of darbepoetin, a direct analogue of EPO also known as NESP ("new erythropoiesis stimulating protein"). Recently a protein conjugate, "synthetic erythropoiesis protein" (SEP), containing precision-length, monodisperse, negatively charged polymers instead of oligosaccharides has...Continue Reading

References

Nov 1, 1995·Medicine and Science in Sports and Exercise·L WideB Ekblom
Jul 26, 1996·Science·J A Wells
May 20, 1999·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·F P BarboneL K Jolliffe
Jun 23, 2000·Nature·F Lasne, J de Ceaurriz
Jun 13, 2001·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·J C Egrie, J K Browne
Jul 5, 2001·The Journal of Gene Medicine·M Cavazzana-CalvoA Fischer
Jan 24, 2002·Journal of the American Chemical Society·Joel GoldbergDale L Boger
May 29, 2002·Experimental Hematology·Dora Ben AlonIlana Nathan
Dec 12, 2002·JONA'S Healthcare Law, Ethics and Regulation·Lynn Smith, Jacqueline Fowler Byers
Feb 8, 2003·Science·Gerd G KochendoerferJames A Bradburne
May 3, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Julie JohnstonJames M Wilson

❮ Previous
Next ❯

Citations

May 23, 2007·Analytical and Bioanalytical Chemistry·Jordi SeguraRicardo Gutiérrez-Gallego
Sep 20, 2012·Endocrine·Leonidas H Duntas, Vera Popovic
Oct 23, 2010·Journal of Applied Physiology·Britt ChristensenJohn J Kopchick
Apr 2, 2009·Organogenesis·Mario Thevis, Wilhelm Schänzer
Jun 10, 2011·Blood·Wolfgang Jelkmann, Carsten Lundby
Mar 8, 2005·Pediatric Clinics of North America·Dilip R PatelEugene F Luckstead
Jun 8, 2007·The Analyst·Mario Thevis, Wilhelm Schänzer
Jan 26, 2017·Molecular and Cellular Endocrinology·Olivier SalaminNicolas Leuenberger
Jun 8, 2018·Drug Testing and Analysis·Gemma Reverter-BranchatJordi Segura
Sep 26, 2007·The Analyst·Hassan M E Azzazy, Mai M H Mansour
Nov 15, 2007·Mass Spectrometry Reviews·Mario ThevisWilhelm Schänzer
Nov 26, 2010·Therapeutic Drug Monitoring·Christina TsitsimpikouAristides Tsatsakis
Nov 12, 2016·Molecular BioSystems·Vladimir N Uversky, Elrashdy M Redwan
Mar 30, 2017·Drug Testing and Analysis·Tessa WilkinNatasha Hamilton
Aug 17, 2005·Journal of Applied Physiology·C CooperD Bienzle
Jun 22, 2018·Frontiers in Physiology·Michael J PuchowiczDavid C Clarke
Sep 26, 2017·Journal of Proteome Research·Montserrat Mancera-ArteuVictòria Sanz-Nebot
Nov 4, 2008·Journal of Chromatography. a·L Franco FraguasM Lönnberg

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Related Papers

Pediatric Clinics of North America
Joseph Congeni, Stephen Miller
British Journal of Sports Medicine
N RobinsonM Saugy
© 2022 Meta ULC. All rights reserved