Recombinant esterase from Corynebacterium pseudotuberculosis in DNA and subunit recombinant vaccines partially protects mice against challenge

Journal of Medical Microbiology
Alexandre Antunes BrumSibele Borsuk

Abstract

We tested the efficacy of the esterase encoded by cp1002_RS09720 from Corynebacteriumpseudotuberculosis in recombinant subunit and DNA caseous lymphadenitis (CLA) vaccines. This target was predicted as one of the best CLA vaccine candidates by mature epitope density analysis. Gene cp1002_RS09720 was cloned into two different vectors (pAE for subunit vaccine and pTARGET for DNA vaccine). Four groups of 15 mice each were immunized with the recombinant esterase rCP09720 associated with aluminium hydroxide adjuvant (G1), pTARGET/cp09720 DNA vaccine (G2), a naked pTARGET (G3) or PBS as a negative control (G4). Immunization occurred in two doses intercalated by a 21 day interval. Twenty-one days after the last dose administration, animals were challenged with a virulent C. pseudotuberculosis MIC-6 strain. G1 showed high levels of IgG1 and IgG2a on days 21 and 42 post-immunization and a significant level of IFN-γ (P<0.05), suggesting a Th1 response. The protection levels obtained were 58.3 and 16.6 % for G1 and G2, respectively. The subunit vaccine composed of the recombinant esterase rCP09720 and Al(OH)3 is a promising antigenic formulation for use against CLA.

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Citations

Jul 10, 2019·Microbial Biotechnology·Thiago Doria BarralRicardo Dias Portela
Nov 21, 2018·Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology·Emma ShardlowChristopher Exley
Jul 28, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Miriam Flores RebouçasRicardo Wagner Portela
Oct 28, 2017·Frontiers in Microbiology·Alberto OliveiraAlice R Wattam
Mar 3, 2021·Applied Microbiology and Biotechnology·Rodrigo Barros de PinhoSibele Borsuk
Mar 7, 2021·Microorganisms·Doglas PariseJan Baumbach

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