Recombinant Expression of a Putative Amidase Cloned from the Genome of Listeria monocytogenes that Lyses the Bacterium and its Monolayer in Conjunction with a Protease

Probiotics and Antimicrobial Proteins
Mustafa SimmonsBruce S Seal

Abstract

Listeria monocytogenes is a Gram-positive, non-spore forming, catalase-positive rod that is a major bacterial food-borne disease agent associated with uncooked meats, including poultry, uncooked vegetables, soft cheeses, and unpasteurized milk. The bacterium may be carried by animals without signs of disease, can replicate at refrigeration temperatures, and is frequently associated with biofilms. There is a need to discover innovative pathogen intervention technologies for this bacterium. Consequently, bioinformatic analyses were used to identify genes encoding lytic protein sequences in the genomes of L. monocytogenes isolates. PCR primers were designed that amplified nucleotide sequences of a putative N-acetylmuramoyl-L-alanine amidase gene from L. monocytogenes strain 4b. The resultant amplification product was cloned into an expression vector, propagated in Escherichia coli Rosetta strains, and the recombinant protein was purified to homogeneity. Gene and protein sequencing confirmed that the predicted and chemically determined amino acid sequence of the recombinant protein designated PlyLM was a putative N-acetylmuramoyl-L-alanine amidase. The recombinant lytic protein was capable of lysing both the parental L. monocytogen...Continue Reading

References

Apr 25, 1988·Nucleic Acids Research·T KristensenW Ansorge
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D F HuntC R Hauer
Jun 12, 1986·Nature·L M SmithL E Hood
Jan 1, 1973·Methods in Enzymology·H D Niall
Jun 23, 2001·The Journal of Antimicrobial Chemotherapy·J M Andrews
Oct 27, 2001·Science·P GlaserP Cossart
Jun 1, 2002·Applied and Environmental Microbiology·D DjordjevicL A McLandsborough
Aug 23, 2002·Nature·Raymond SchuchVincent A Fischetti
Aug 7, 2003·Applied and Environmental Microbiology·Britta LeverentzAlexander Sulakvelidze
Oct 24, 2003·Infection and Immunity·Jutta M LoefflerVincent A Fischetti
Oct 25, 2003·Antimicrobial Agents and Chemotherapy·Julie A WuJohn F Kokai-Kun
Dec 9, 2003·Applied and Environmental Microbiology·Monica K BoruckiDouglas R Call
Jul 17, 2004·Microbiology·David G PritchardJeffrey A Engler
Aug 7, 2004·Journal of Clinical Microbiology·Michel DoumithPaul Martin
May 18, 2005·Journal of Food Protection·M E BerrangL L Genzlinger
Aug 17, 2005·The Journal of Biological Chemistry·Lieh Yoon LowRobert C Liddington
Aug 30, 2005·Trends in Microbiology·Vincent A Fischetti
Oct 26, 2005·Nature Reviews. Drug Discovery·Carl Nathan, Frederick M Goldberg
Apr 7, 2006·Applied and Environmental Microbiology·David M DonovanDavid G Pritchard
Jun 10, 2006·Archives of Microbiology·Magdalena Popowska, Zdzislaw Markiewicz
Jul 6, 2006·Applied and Environmental Microbiology·Yuelian ShenArvind A Bhagwat
Oct 3, 2006·International Journal of Food Microbiology·Megha Gandhi, Michael L Chikindas
Nov 7, 2006·Applied and Environmental Microbiology·Peter Sass, Gabriele Bierbaum
Aug 28, 2007·Microbes and Infection·Carmen Buchrieser
Aug 28, 2007·Microbes and Infection·Bala Swaminathan, Peter Gerner-Smidt
Feb 13, 2008·FEMS Microbiology Reviews·Waldemar VollmerSimon Foster
May 13, 2008·The Journal of Infectious Diseases·Denis GrandgirardStephen L Leib
May 24, 2008·Science·Anders F Andersson, Jillian F Banfield
Jun 17, 2008·International Journal of Food Microbiology·Aurélie RieuPascal Piveteau
Aug 12, 2008·Current Pharmaceutical Biotechnology·J Azeredo, I W Sutherland

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Citations

Oct 4, 2012·Future Microbiology·Mathias SchmelcherMartin J Loessner
Dec 29, 2015·Current Opinion in Biotechnology·Mathias Schmelcher, Martin J Loessner
Mar 4, 2020·Virologica Sinica·Marzanna Łusiak-SzelachowskaAndrzej Górski
Aug 25, 2020·Cancer Biotherapy & Radiopharmaceuticals·Mohammad-Taghi Bahreyni-ToossiFereshteh Vaziri Nezamdoust
Apr 19, 2018·Frontiers in Microbiology·Jessica A GrayEdward M Fox

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