Recombinant expression of a truncated capsid protein of beak and feather disease virus and its application in serological tests

Avian Pathology : Journal of the W.V.P.A
Reimar JohneHermann Müller

Abstract

Beak and feather disease virus (BFDV) causes severe disease characterized by irreversible feather disorders and severe immunosuppression in many psittacine species. BFDV cannot be propagated in tissue or cell cultures, rendering virus propagation and thus diagnosis rather difficult. To develop reliable diagnostic methods, the region encoding the BFDV capsid protein C1 was cloned from an infected sulphur-crested cockatoo (Cacatua galerita). Phylogenetic analysis showed this gene had 76.3 to 83.2% amino acid identity to published sequences. No protein was detected after induction of full-length C1 expression in Escherichia coli. However, deletion of an amino-terminal arginine-rich sequence facilitated expression. C1(39-244)-His, a polyhistidine-tailed variant of this protein, was purified and used for immunization of chickens. The immune sera detected C1 with an apparent molecular weight of 27 kDa in western blots of organ homogenates of BFDV-infected birds. Using C1(39-244)-His as antigen, 11 psittacine sera were tested for the presence of BFDV-specific antibodies by enzyme-linked immunosorbent assay and immunoblotting. The results obtained correlated well with the BFDV-specific haemagglutination inhibition activity of the sera,...Continue Reading

References

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Citations

Mar 16, 2006·Avian Pathology : Journal of the W.V.P.A·Dave Cavanagh
Sep 17, 2010·Expert Review of Molecular Diagnostics·Vinayagamurthy BalamuruganRaj Kumar Singh
Apr 23, 2010·Veterinary Microbiology·Shao-Ning LiuShi-Jin Jiang
May 16, 2009·Journal of Virological Methods·Patrick L ShearerShane R Raidal
May 12, 2009·Journal of Virological Methods·Patrick L ShearerShane R Raidal
Jul 3, 2009·The Journal of General Virology·Karyna RosarioMya Breitbart
Feb 14, 2013·Journal of Virological Methods·Edward I PattersonShane R Raidal
Aug 13, 2013·Biochemical and Biophysical Research Communications·Edward I PattersonJade K Forwood
Oct 23, 2008·Avian Diseases·Volker SchmidtMaria-Elisabeth Krautwald-Junghanns
Oct 21, 2017·Archives of Virology·Chia-Fang HoChi-Young Wang
Apr 11, 2006·The Journal of General Virology·Reimar JohneHermann Müller

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Datasets Mentioned

BETA
AY345131
AF311301

Methods Mentioned

BETA
PCR
electrophoresis
protein assay
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

Lasergene
Clustal W
Pro Analyzer
Gel

Related Concepts