Recombinant expression of bioactive peptide lunasin in Escherichia coli

Applied Microbiology and Biotechnology
Chin-Feng Liu, Tzu-Ming Pan

Abstract

Lunasin, a cancer-preventive peptide, was isolated from soybean, barley, and wheat. Previous studies showed that this 43-amino acid peptide has the ability to suppress chemical carcinogen-induced transformation in mammalian cells and skin carcinogenesis in mice. In this study, we attempted to use the Escherichia coli T7 expression system for expression of lunasin. The lunasin gene was synthesized by overlapping extension polymerase chain reaction and expressed in E. coli BL21(DE3) with the use of vector pET29a. The recombinant lunasin containing his-tag at the C-terminus was expressed in soluble form which could be purified by immobilized metal affinity chromatography. After 4 h, the expression level is above 4.73 mg of recombinant his-tagged lunasin/L of Luria-Bertani broth. It does not affect the bacterial growth and expression levels. This is the first study that successfully uses E. coli as a host to produce valuable bioactive lunasin. The result of in vitro bioassay showed that the purified recombinant lunasin can inhibit histone acetylation. Recombinant lunasin also inhibits the release of pro-inflammatory cytokines (tumor necrosis factor-alpha, interleukin-1beta, and nitric oxide production). Compared with other research...Continue Reading

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Citations

Mar 2, 2012·Microbial Cell Factories·Stuart KyleMichael J McPherson
Apr 15, 2014·Journal of Proteomics·Margarita Ortiz-MartinezSilverio García-Lara
Jan 30, 2015·Journal of Food Science·Vaibhao Kisanrao LuleSudhir Kumar Tomar
Sep 13, 2014·Biotechnology Letters·Omar González-OrtegaRuth Elena Soria-Guerra
Mar 15, 2014·Experimental Biology and Medicine·Gillian Jones, Ajay Srivastava
Oct 11, 2017·Journal of the Science of Food and Agriculture·Chia-Chien HsiehBlanca Hernández-Ledesma
Sep 20, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Guixing RenGang Zhao
Apr 10, 2019·Neural Regeneration Research·Prajakta DeshpandeAmit Singh

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