Recombinant expression of thermostable processive MtEG5 endoglucanase and its synergism with MtLPMO from Myceliophthora thermophila during the hydrolysis of lignocellulosic substrates

Biotechnology for Biofuels
Anthi KarnaouriPaul Christakopoulos

Abstract

Filamentous fungi are among the most powerful cellulolytic organisms in terrestrial ecosystems. To perform the degradation of lignocellulosic substrates, these microorganisms employ both hydrolytic and oxidative mechanisms that involve the secretion and synergism of a wide variety of enzymes. Interactions between these enzymes occur on the level of saccharification, i.e., the release of neutral and oxidized products, but sometimes also reflected in the substrate liquefaction. Although the synergism regarding the yield of neutral sugars has been extensively studied, further studies should focus on the oxidized sugars, as well as the effect of enzyme combinations on the viscosity properties of the substrates. In the present study, the heterologous expression of an endoglucanase (EG) and its combined activity together with a lytic polysaccharide monooxygenase (LPMO), both from the thermophilic fungus Myceliophthora thermophila, are described. The EG gene, belonging to the glycoside hydrolase family 5, was functionally expressed in the methylotrophic yeast Pichia pastoris. The produced MtEG5A (75 kDa) featured remarkable thermal stability and showed high specific activity on microcrystalline cellulose compared to CMC, which is indi...Continue Reading

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Citations

Nov 19, 2017·Applied Biochemistry and Biotechnology·Luana Parras MeleiroRosa Prazeres Melo Furriel
May 14, 2020·The Journal of Biological Chemistry·Sophanit MekashaVincent G H Eijsink
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May 18, 2021·Bioresource Technology·Reeta Rani SinghaniaCheng Di Dong
Aug 10, 2021·International Journal of Biological Macromolecules·Anica DadwalTulasi Satyanarayana

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Datasets Mentioned

BETA
AFY52522
ACB06750

Methods Mentioned

BETA
X-ray
PCR
flow filtration
electrophoresis
Gel Filtration
protein assay

Software Mentioned

ExPASy
Thermocline
ProtParam
NetOGlyc
Chromeleon
SignalP
NetNGlyc

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