PMID: 16613564Apr 15, 2006Paper

Recombinant expression systems for allergen vaccines

Inflammation & Allergy Drug Targets
Mohan B Singh, Prem L Bhalla

Abstract

Allergen immunotherapy of future is likely to be based on allergy vaccines that contain engineered allergens modified to abolish or substantially reduce their IgE-binding activity in order to remove the risk of unwanted anaphylactic responses. The development of efficient systems for the production of recombinant allergens in sufficient quantities is requirement for establishing use of engineered allergens as components of allergy vaccines. This review outlines relative advantages and disadvantages of various heterologous systems for production of recombinant allergens. Microbial systems are most convenient and cost effective platforms for the production of recombinant allergens. However, lack of post-translational processing implies that some allergens have to be expressed in eukaryotic systems for proper folding and post-translational modifications such as glycosylation. Yeast systems can yield high levels of recombinant allergens but often are associated with hyper- glycosylation problems. Mammalian cell culture systems offer suitable post -translational modifications but are nearly hundred fold more expensive than microbial systems. The use of plants as bio-factories for production of recombinant allergens is emerging as a ...Continue Reading

Citations

Feb 14, 2008·Biotechnology Letters·Marcie H MoehnkeChristopher M Kearney
Mar 14, 2013·Allergologia et immunopathologia·R FalakM Sankian
Jan 15, 2013·Biologicals : Journal of the International Association of Biological Standardization·Xuan ShenMark S Galinski
Sep 26, 2008·Science in China. Series C, Life Sciences·LiQiong GuoJunFang Lin
Aug 8, 2013·Methods : a Companion to Methods in Enzymology·Mayte VillalbaEva Batanero
Apr 9, 2008·FEMS Microbiology Reviews·Ai-Sheng XiongQuan-Hong Yao
Oct 1, 2008·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Georg SchmidtFatima Ferreira
May 30, 2021·Molecular Immunology·Azahara González-RuizJerónimo Carnés

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