PMID: 9182704Jun 1, 1997Paper

Recombinant fish neurotrophin-6 is a heparin-binding glycoprotein: implications for a role in axonal guidance

The Biochemical Journal
X LiR Götz

Abstract

Neurotrophin-6 (NT-6) was identified in the teleost fish Xiphophorus as a new member of the neurotrophin gene family. NT-6 binds specifically the glycosaminoglycan heparin. In this study NT-6 was expressed in a stably transfected mammalian cell line, and in insect cells via a recombinant baculovirus. It was purified to homogeneity and characterized by MS and N-terminal sequencing. NT-6 from both expression systems was proteolytically processed at one of two protease cleavage motifs and was found to be glycosylated. It supported the survival of embryonic chick sensory neurons; half-maximal survival was observed at 100 ng/ml. Furthermore, NT-6 elicited neurite outgrowth in explanted embryonic dorsal root ganglia. Addition of heparin into the medium did not potentiate the activity of NT-6 in survival assays. However, when a sensory ganglion explant was cultured in a collagen gel matrix assay adjacent to a heparin bead coated with NT-6, neurite outgrowth directed towards the bead was observed. This indicated that NT-6 was slowly released from the heparin bead generating a concentration gradient of NT-6 instrumental for axonal guidance in vitro. Thus the interaction of NT-6 with heparin might not be required for the activation of th...Continue Reading

Citations

Dec 17, 2004·Journal of the American Society of Nephrology : JASN·Nadia Abdel WahabRoger M Mason
Apr 9, 2005·Microscopy Research and Technique·Nadia ArcamonePaolo De Girolamo
Apr 1, 1999·Archives of Physiology and Biochemistry·I D NagtegaalE Marani
Sep 29, 2004·Journal of Neurobiology·Derek N AdamsPaul C Letourneau
Mar 10, 2000·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·G Heinrich, T Lum
Aug 7, 1998·Brain Research. Developmental Brain Research·G K SeaboldM W Miller
Jan 19, 2006·Current Opinion in Neurobiology·David Van VactorKarl G Johnson

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